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Questions related from Stephen Karori
Which is the best solution for diluting DMSO during extraction of plant compounds for cells culture assay? Can one use PBS or it must be ddH20?
07 July 2013 1,481 6 View
Is it possible to view mRNA on agarose gel? If one uses aligo dTmagnetic beads specific for mRNA, what kind of gel image should one get?
07 July 2013 2,275 9 View
I got the results seen attached below before (up) and after (down) treating cells with plant extract. Can somebody help me understand why the treated cells have spaces within the monolayer. Does...
06 June 2013 4,694 2 View
Can the 4T1 metastatic breast cancer model - which is a syngeneic xenograft model based on 4T1-12B - be used as a model to study the anticancer effects of plant based compounds without injecting...
06 June 2013 6,871 16 View
06 June 2013 2,657 8 View
I am planning to add a plant extract into cells then harvest and carry out molecular assays. How do I treat my plant extracts before adding to the flask containing cells? Is it by using...
05 May 2013 7,918 24 View
I have cells at near confluence which I plan to harvest soon. A density of 2.0x10power5 is needed for RNA harvest. What amount is suitable for the assay (RNA harvest) at that density? Secondly,...
05 May 2013 5,835 6 View
