Compared to humans, what are the metabolic (biochemical) features and differences ?
What is important when we study the metabolic of mice and want to extrapolate to humans ?
Since the drug quantity given to mice depends on the animal weight: 1. How often we have to weigh the animal to adjust? Once a week? 2. Is it acceptable if in the same group that has around 8 mice...
09 October 2014 9,218 5 View
For a pharmacological and another pathway study related to Alzheimer's disease (such as those including building animal models), what is the best animal choice? The mice or the rats? If it is the...
08 September 2014 7,627 6 View
To study Alzheimer's disease , I need to study the involvement of APOE genes in Amyloid Beta production thus, I am searching for suitable cell lines that specifically express these genes (APOE 2,...
08 September 2014 2,643 2 View
When we have a therapeutic dose of a compound and also its LD measured in rats can we use it when we design an experiment on mice ? (And vice versa)
08 September 2014 2,097 10 View
If we have to select an animal to study either the effect of some drugs on Alzheimer's disease what would be the best choice ? Mice ? Rats ? Others ? Why ?
08 September 2014 2,220 4 View
See above
07 August 2014 939 1 View
I plan to investigate the implications of a compound in Alzheimer's disease and conduct some pharmacological tests. What is the cell line(s) you suggest me to use?
07 August 2014 2,010 8 View
What are the est cell lines to use to study AD in cell cultures (In vitro)?
07 August 2014 5,344 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View