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Questions related from Duco Kramer
Dear researchers We are preparing for superresolution microscopy. I read some papers where the cells are prepared with the use of a special cytoskeletal buffer (CB) and followed by NaBH4...
02 February 2019 2,655 5 View
I want to stain an intracellular protein in 4% FA fixed cultured cells. I have to permeabilise the cells. Triton is to harsh (The ultra structure is too much damaged for EM). I need a milder...
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Dear RGs I wonder if the German manufactor of ultra microtome knives dEYEmond is still alive? The website of this firm and it's 'mother' SciMed is not fully functional, no responce to mail and...
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After consulting a colleague I tried to produce sodium ethoxide by mixing NaOH in 96% EtOH. I tried to do so and ended up with a turbid white solution. An earlier question on RG revealed that I...
01 January 2016 3,517 8 View
We would like to use sodium ethoxide solution for etching EPON and LR-white EM sections. Today I started making the saturated sodium ethoxide solution. I put 13 g NaOH in 60 ml 96% EtOH. Actually...
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I just realized that a grid for EM is quite expensive. Ni and Cu grids (one-hole in my case) cost around €0,5 each ($0,7). I have tried with overnight chloroform bu that was not satisfactory. Does...
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