I plan to investigate the implications of a compound in Alzheimer's disease and conduct some pharmacological tests.
What is the cell line（s） you suggest me to use?
Hi Abdelaziz,
It all boils down to what aspect of AD are you interested to look at? Amyloid toxicity? oxidative stress? mitochondrial dysfunction? etc. There are different cell models for different aspects of AD.
There are several transfected models used for AD investigations. For e.g. APP stably-transfected cell lines are suitable for amyloid toxicity investigation. So far the APP cell line that I looked at consistently showed Abeta accumulation, and more interestingly, a large extent of impaired glucose utilisation and mitochondrial dysfunction. I won't say it is the best model available, but it covers quite a lot of different aspects in AD, and is a neat model for repeated screening and testing as they grow robustly.
You also have primary cell lines (either harvested from transgenic animal or clinical samples taken from human patients), but generally these cells do not grow well, and it is difficult to interpret pharmacological data based on these cell lines.
On the other hand, you have chemically induced model. Most of these models replicate the oxidative stress and in some cases, the immunological aspects of AD. These models are often seen as "acute" toxicity model as the disease-causing agents were introduced externally, skipping the whole upstream processes that led to production of these toxic agents in the model itself. Nevertheless, when used carefully, they still represent useful models for screening of therapeutic compounds.
I am sure there are a lot more great models out there. For e.g. one relatively new one is induced pluripotent stem cells (iPS) that are now available for AD research. It is worth thinking about that you are interested to investigate, and then look for a model that is suitable in answering your research question. Good luck.
Dear Abdelaziz
It is better for you to choose your cell line based on your desired gene instead of disease type. It seems that AD is a complex disease.
Hi Abdelaziz,
It all boils down to what aspect of AD are you interested to look at? Amyloid toxicity? oxidative stress? mitochondrial dysfunction? etc. There are different cell models for different aspects of AD.
There are several transfected models used for AD investigations. For e.g. APP stably-transfected cell lines are suitable for amyloid toxicity investigation. So far the APP cell line that I looked at consistently showed Abeta accumulation, and more interestingly, a large extent of impaired glucose utilisation and mitochondrial dysfunction. I won't say it is the best model available, but it covers quite a lot of different aspects in AD, and is a neat model for repeated screening and testing as they grow robustly.
You also have primary cell lines (either harvested from transgenic animal or clinical samples taken from human patients), but generally these cells do not grow well, and it is difficult to interpret pharmacological data based on these cell lines.
On the other hand, you have chemically induced model. Most of these models replicate the oxidative stress and in some cases, the immunological aspects of AD. These models are often seen as "acute" toxicity model as the disease-causing agents were introduced externally, skipping the whole upstream processes that led to production of these toxic agents in the model itself. Nevertheless, when used carefully, they still represent useful models for screening of therapeutic compounds.
I am sure there are a lot more great models out there. For e.g. one relatively new one is induced pluripotent stem cells (iPS) that are now available for AD research. It is worth thinking about that you are interested to investigate, and then look for a model that is suitable in answering your research question. Good luck.
I would not recommend to use cell lines, to start with. In cells lines (CHO, in the case) you can test for amyloid-beta production but I won't test any downstream effect of abeta. If you want to test for abeta production/secretion, than CHO stably expressing preselinin and mutant app are available....
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