I need a certain method to cause irreversible DNA denaturation in double stranded FIsh DNA?

I need a certain method to cause irreversible DNA denaturation in double stranded FIsh DNA

01 February 2019 6,717 3 View

Dose equivalent to niosome encapsulation dose?

I'm preparing niosome encapsulating a certain natural extract how can I caclulate the encapsulated dose which is equivalent to the free dose of that substance?

11 December 2018 7,655 3 View

Animal dosage from mgs to mls?

I want to treat rats with a certain drug loaded on a nanocarrier with a certain dose... The problem the i have.. That when converting my formulation to powder through air drying.. The yield is...

01 February 2018 7,203 3 View

Total proteins extraction?

I want a simple protocol ( non-costly) for the separation of total proteins from breast tissues without altering their conformations

01 February 2018 5,784 1 View

Total proteins extraction?

Would someone suggest a simple protocol for total proteins extraction from human tissues that don't alter protein conformation ?

01 February 2018 3,006 2 View

Best solvent for Thymoquinone?

What is the best solvent for IP injection in rats for TQ for one week treatment ?

01 February 2018 1,059 1 View

Best solvent for chitosan nanoparticles ?

what is the best solvent for chitosan nanoparticles for IP injection in rats for one week treatment ?

01 February 2018 7,361 2 View

DNA extraction protocol ?

Would someone tell me a successful simple protocol for DNA isolation from animal tissues , that gives reasonable yield and which is not costly and does not alter DNA structure ?

01 February 2018 8,545 7 View

Cel membrane isolation?

What is the simple organism or cell type from which I can isolate its membrane and what is the best model to study the interaction of nanoparticles with cell membrane ?

31 December 2017 4,319 3 View

Encapsulated drug dose calculation?

I will make an in vivo experiment in which I want to compare between the effect of a free drug and the same drug loaded on chitosan nanoparticles.. how can I calculate the dose of nanoparticles...

31 December 2017 4,358 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View