I need a certain method to cause irreversible DNA denaturation in double stranded FIsh DNA?

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Dose equivalent to niosome encapsulation dose?

I'm preparing niosome encapsulating a certain natural extract how can I caclulate the encapsulated dose which is equivalent to the free dose of that substance?

11 December 2018 7,710 3 View

Animal dosage from mgs to mls?

I want to treat rats with a certain drug loaded on a nanocarrier with a certain dose... The problem the i have.. That when converting my formulation to powder through air drying.. The yield is...

01 February 2018 7,258 3 View

Total proteins extraction?

I want a simple protocol ( non-costly) for the separation of total proteins from breast tissues without altering their conformations

01 February 2018 5,835 1 View

Total proteins extraction?

Would someone suggest a simple protocol for total proteins extraction from human tissues that don't alter protein conformation ?

01 February 2018 3,058 2 View

Best solvent for Thymoquinone?

What is the best solvent for IP injection in rats for TQ for one week treatment ?

01 February 2018 1,114 1 View

Best solvent for chitosan nanoparticles ?

what is the best solvent for chitosan nanoparticles for IP injection in rats for one week treatment ?

01 February 2018 7,408 2 View

DNA extraction protocol ?

Would someone tell me a successful simple protocol for DNA isolation from animal tissues , that gives reasonable yield and which is not costly and does not alter DNA structure ?

01 February 2018 8,606 7 View

Encapsulated drug dose calculation?

I will make an in vivo experiment in which I want to compare between the effect of a free drug and the same drug loaded on chitosan nanoparticles.. how can I calculate the dose of nanoparticles...

31 December 2017 4,413 2 View

Cel membrane isolation?

What is the simple organism or cell type from which I can isolate its membrane and what is the best model to study the interaction of nanoparticles with cell membrane ?

31 December 2017 4,375 3 View

Tween-coated chitosan?

I prepared TPP chitosan and loaded it with thymoquinone drug, the zeta potential was about 30 mv , when I made coat with tween 80 to this combination, zeta potential dropped to 4 Isa there any...

31 December 2017 8,664 0 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

Do anyone knows any book dealing with fish growth/zootechnical performances and somatic indexes in aquaculture?

In the aquaculture and fish nutrition research field, a number of growth and somatic indexes are often used, including specific growth rate (SGR), feed conversion rate (FCR), protein efficiency...

22 July 2024 2,480 4 View

Why do fish have fishy odors naturally ?

Chemical Engineering /Food Engineering

20 July 2024 4,152 4 View

Chromosomal spreads are shrunken after fluorescence in situ hybridization (FISH), why?

Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...

08 July 2024 8,687 3 View

Determination of fatty acids in fish?

I measured the fatty acids in the fish, using the internal standard method in the national standard method, I first extracted the fish oil with cable extraction, please ask me how many grams of...

02 July 2024 900 1 View

How many fish otoliths and charophyte gyrogonites should you find in a sedimentary sample weighing 50g in order to be considered enviromental indices?

It's for my thesis on Quaternary costal sediments from Western Greece.

01 July 2024 4,854 0 View

Does anyone knows about any protandrous fish inhabiting the Mediterranean Sea, smaller than 20 cm (adult) and that change sex relatively quickly?

Other than Sparids

27 June 2024 1,809 0 View

Fish physiology and biochemistry journal ?

No response from fish physiology and biochemistry journal after a year of peer review. Journal editorial team is not responding even for Request for withdrawal of our manuscript. We are struck...

25 June 2024 8,918 0 View

How to properly label fish feed pellets with stable isotopes for tracing C and N flow?

Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...

19 June 2024 746 0 View

What are those vacuolated structures in my tilapia intestinal cross-sections (H&E stained)? Do the sections exhibit signs of enteritis?

Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...

15 June 2024 3,208 4 View

Ahmed Housanie Elsappah

hi , Dr. Heba Mohamed Fahmy

i suggest you read these:-

1- Characterization of denaturation and renaturation of DNA for DNA hybridization

Article Characterization of denaturation and renaturation of DNA for...

2- Influence of Formamide on the Thermal Stability of DNA

Article Influence of Formamide on the Thermal Stability of DNA

3- The Reversible Denaturation of Deoxvribonucleic Acid-deuendent Ribonucleic Acid Polymerase of Escherichia coZi*

http://www.jbc.org/content/249/17/5394.full.pdf

4- Epi-fluorescence Microscopy of Single Molecule DNA Denaturation in situ

https://www.biorxiv.org/content/biorxiv/early/2018/07/13/368423.full.pdf

5- NUCLEIC ACID LABELING AND DETECTION SYSTEMS

https://vectorlabs.com/media/docs/brochures/MBB.pdf

6- Tracking Multiple Genomic Elements Using Correlative CRISPR Imaging and Sequential DNA FISH

https://www.cell.com/biophysj/pdfExtended/S0006-3495(17)30162-5

good luck

Heba Mohamed Fahmy

Amany M. Hamad

Shahbaa Khalil

Interest for answers