I want a simple protocol ( non-costly) for the separation of total proteins from breast tissues without altering their conformations
Article Optimized Protocol for Protein Extraction from the Breast Ti...
I need a certain method to cause irreversible DNA denaturation in double stranded FIsh DNA
01 February 2019 6,800 3 View
I'm preparing niosome encapsulating a certain natural extract how can I caclulate the encapsulated dose which is equivalent to the free dose of that substance?
11 December 2018 7,710 3 View
I want to treat rats with a certain drug loaded on a nanocarrier with a certain dose... The problem the i have.. That when converting my formulation to powder through air drying.. The yield is...
01 February 2018 7,258 3 View
Would someone suggest a simple protocol for total proteins extraction from human tissues that don't alter protein conformation ?
01 February 2018 3,058 2 View
What is the best solvent for IP injection in rats for TQ for one week treatment ?
01 February 2018 1,114 1 View
what is the best solvent for chitosan nanoparticles for IP injection in rats for one week treatment ?
01 February 2018 7,408 2 View
Would someone tell me a successful simple protocol for DNA isolation from animal tissues , that gives reasonable yield and which is not costly and does not alter DNA structure ?
01 February 2018 8,606 7 View
I will make an in vivo experiment in which I want to compare between the effect of a free drug and the same drug loaded on chitosan nanoparticles.. how can I calculate the dose of nanoparticles...
31 December 2017 4,413 2 View
What is the simple organism or cell type from which I can isolate its membrane and what is the best model to study the interaction of nanoparticles with cell membrane ?
31 December 2017 4,375 3 View
I prepared TPP chitosan and loaded it with thymoquinone drug, the zeta potential was about 30 mv , when I made coat with tween 80 to this combination, zeta potential dropped to 4 Isa there any...
31 December 2017 8,664 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View