23 Questions 7 Answers 0 Followers
Questions related from Heba Mohamed Fahmy
I need a certain method to cause irreversible DNA denaturation in double stranded FIsh DNA
02 February 2019 6,778 3 View
I'm preparing niosome encapsulating a certain natural extract how can I caclulate the encapsulated dose which is equivalent to the free dose of that substance?
12 December 2018 7,687 3 View
Would someone tell me a successful simple protocol for DNA isolation from animal tissues , that gives reasonable yield and which is not costly and does not alter DNA structure ?
02 February 2018 8,578 7 View
I want to treat rats with a certain drug loaded on a nanocarrier with a certain dose... The problem the i have.. That when converting my formulation to powder through air drying.. The yield is...
02 February 2018 7,238 3 View
I want a simple protocol ( non-costly) for the separation of total proteins from breast tissues without altering their conformations
02 February 2018 5,820 1 View
Would someone suggest a simple protocol for total proteins extraction from human tissues that don't alter protein conformation ?
02 February 2018 3,039 2 View
what is the best solvent for chitosan nanoparticles for IP injection in rats for one week treatment ?
02 February 2018 7,393 2 View
What is the best solvent for IP injection in rats for TQ for one week treatment ?
02 February 2018 1,096 1 View
I will make an in vivo experiment in which I want to compare between the effect of a free drug and the same drug loaded on chitosan nanoparticles.. how can I calculate the dose of nanoparticles...
01 January 2018 4,392 2 View
What is the simple organism or cell type from which I can isolate its membrane and what is the best model to study the interaction of nanoparticles with cell membrane ?
01 January 2018 4,360 3 View
How can I interpret the decrease in the size measured by DLS for tween coated chitosan , from the uncoated formula?
01 January 2018 6,306 0 View
I prepared TPP chitosan and loaded it with thymoquinone drug, the zeta potential was about 30 mv , when I made coat with tween 80 to this combination, zeta potential dropped to 4 Isa there any...
01 January 2018 8,650 0 View
I was testing the cytotoxicity of a nano agent of cancerous cell, it showed cytotoxicity in dose dependent manner, it shows also DNA damage (comet assay) however, no oxidative stress is exhibited...
11 November 2017 7,642 3 View
after the IV injection of rats with magnetic iron oxide nanoparticles, I evaluated the iron concentration per gram tissue in each brain area, I found a significant decrease in the iron levels in...
08 August 2017 9,021 1 View
I want to make ICP for nanometal detection in biological tissues, the first step is the tissue digestion, is there is any easy protocol or method to follow it ?
04 April 2017 6,997 0 View
I want to know what is the best method to prepare florescent DPPC liposomes using rhodamine B dye ? At what step of liposome preparation should the dye be used ? and how can I photograph the...
12 December 2016 3,196 2 View
I need to know the cheapest method for nanoparticles synthesis
10 October 2015 1,115 3 View
what is the aprroximate price of a western blot unit containning: - Protein electrophoresis apparatus -transfer apparatus (tank method ) --Odysee scanner (IR scanner)
03 March 2015 5,219 1 View
The proteins and lipids of the cytoplasm have less surface tension, hence they are found at the surface forming the membrane. On the other hand the chemical substances (NaCl, etc.) have high...
03 March 2015 6,092 0 View
are the kits used for measuring TSH and FSH levels in humans are the same as rats ? Are there using Elisa ?
02 February 2015 5,385 0 View
What is the best way or technique to measure the concentration of 20 nm gold nanoparticles per gram of brain areas tissues ?
01 January 2015 5,660 9 View
i need a good place from which i can purchase AMH elisa kits for rats and the approximate price plz, i live in Egypt.
12 December 2014 5,959 3 View
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01 January 2014 4,740 0 View