I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the interpolated concentrations for my unknown samples different between the two standards?
Alireza Naderi Sohi
Dear Alaleh
If you are sure of the 100% purity of your homemade standard sample and the absence of interfering impurity in it, and the dilution is also done with the special buffer of the kit, please show the linear equation of the two standard graphs. Is the difference between the two graphs only in the y-intercept or are the slope of the lines also different?
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