Some more information about your experiment would be helpful: From what tissue do you want to harvest lymphocytes? Is it mouse tissue? The choice of isolation and culture methods will also partially depend on what you intend to do with the cultured lymphocytes - What kind of experiment are you planning?

Hey

attached  Primary cell culture Handbook

I think it will help you

Best regards

Thank you si much Issa.

Schabla I want to isolate limphocytes from Adenoids and than i want to grow. 

HI to everybody,

I want to  coltured Lymphocytes isolated from Adenoids or Tonnsilis. Somebody link me the best protocol and the medium that i must used to growing it?

Thank for halp.