3D spheroids and count?

Hi, I have a question about the count of 3D spheroids. How can I count the number of spheroids starting from the number of seeded cells? After 3 days colture I count spheroids that have 100 um...

02 March 2018 5,807 3 View

Study tumour haeterogenity

Hello,Can anyone suggest a bioinformatic approach to studying tumor heterogeneity in tumor samples? In other words, I performed exome analysis on my samples and now I want to build an evolutionary...

06 July 2017 7,989 1 View

How can evaluate the activity of inverseTrascriptase on a fresh sample?

Hi to all. someone knows or can suggest me a kit to evaluate the activities of inversetrascriptase the cell sample isolated from fresh tissue? Thanks so much

10 November 2016 4,595 4 View

Do you have a protocol to select Matrigel and prepare conditioned media to growing breast organoids?

I read some articles about organoids and their isolation from Frash fabric, but I have no idea and experience on how to select Matrigel and the average condition for growing and keeping them in...

09 October 2016 5,618 3 View

Cultured human lymphocytes and virus stimulation?

Hi to all, I m started to grow lymphocytes Which cultures are positive to one sequences of the virus. Now, if I want to stimulate the production of the virus how can i do it? thanks for helping.

09 October 2016 4,030 1 View

PCR to detecte fragment of betaretrovirus sequence?

I m trying to amplified fragment of one betaretrovirus using primer which are designed on mouse sequence. I amplified something but when I extracted band on gel and sequenced that i don't read the...

09 October 2016 9,186 4 View

The best method to isolate and grow lymphocytes from fresh tessue?

Hello to all, i m starting to work with a culture of lymphocytes , but I want to know which is the best method for isolating these from tessue fresh surgery. Thanks for the help.

06 July 2016 1,461 4 View

Herceptin 150mg for Infusion is appropriate for Cell Culture?

03 April 2016 3,445 4 View

How can I confirm the mutation in PMS2 gene obtained through NGS?

03 April 2016 8,412 10 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View