Prospero Civita

10 Questions 12 Answers 0 Followers

Questions related from Prospero Civita

FFPE RNA Seq Data?

Hi all, can anyone please help me to find dataset of Normal BRain FFPE trascriptomic data that i can use as a control i my experiment? Thank you for helping.

09 September 2018 141 0 View

3D spheroids and count?

Hi, I have a question about the count of 3D spheroids. How can I count the number of spheroids starting from the number of seeded cells? After 3 days colture I count spheroids that have 100 um...

03 March 2018 5,842 3 View

Study tumour haeterogenity

Hello,Can anyone suggest a bioinformatic approach to studying tumor heterogeneity in tumor samples? In other words, I performed exome analysis on my samples and now I want to build an evolutionary...

07 July 2017 8,022 1 View

How can evaluate the activity of inverseTrascriptase on a fresh sample?

Hi to all. someone knows or can suggest me a kit to evaluate the activities of inversetrascriptase the cell sample isolated from fresh tissue? Thanks so much

11 November 2016 4,621 4 View

Do you have a protocol to select Matrigel and prepare conditioned media to growing breast organoids?

I read some articles about organoids and their isolation from Frash fabric, but I have no idea and experience on how to select Matrigel and the average condition for growing and keeping them in...

10 October 2016 5,644 3 View

PCR to detecte fragment of betaretrovirus sequence?

I m trying to amplified fragment of one betaretrovirus using primer which are designed on mouse sequence. I amplified something but when I extracted band on gel and sequenced that i don't read the...

10 October 2016 9,214 4 View

Cultured human lymphocytes and virus stimulation?

Hi to all, I m started to grow lymphocytes Which cultures are positive to one sequences of the virus. Now, if I want to stimulate the production of the virus how can i do it? thanks for helping.

10 October 2016 4,058 1 View

The best method to isolate and grow lymphocytes from fresh tessue?

Hello to all, i m starting to work with a culture of lymphocytes , but I want to know which is the best method for isolating these from tessue fresh surgery. Thanks for the help.

07 July 2016 1,487 4 View

How can I confirm the mutation in PMS2 gene obtained through NGS?

Exome data obtained from NGS analysis show the mutation in PMS_2 gene. I would like to confirm the mutation by Sanger sequencing but the PMS2 have a Pseudogene PMS2CL. I'm trying to isolate the...

04 April 2016 8,441 10 View

Herceptin 150mg for Infusion is appropriate for Cell Culture?

I would like to test the effect of Trastuzumab (ROCHE) on BT474 cells, but I have only Herceptin for infuison. Is it possible to use it for cell cultures? I have to resusped it in the classic...

04 April 2016 3,474 4 View