FFPE RNA Seq Data?

Hi all, can anyone please help me to find dataset of Normal BRain FFPE trascriptomic data that i can use as a control i my experiment? Thank you for helping.

08 September 2018 111 0 View

3D spheroids and count?

Hi, I have a question about the count of 3D spheroids. How can I count the number of spheroids starting from the number of seeded cells? After 3 days colture I count spheroids that have 100 um...

02 March 2018 5,807 3 View

How can evaluate the activity of inverseTrascriptase on a fresh sample?

Hi to all. someone knows or can suggest me a kit to evaluate the activities of inversetrascriptase the cell sample isolated from fresh tissue? Thanks so much

10 November 2016 4,595 4 View

Do you have a protocol to select Matrigel and prepare conditioned media to growing breast organoids?

I read some articles about organoids and their isolation from Frash fabric, but I have no idea and experience on how to select Matrigel and the average condition for growing and keeping them in...

09 October 2016 5,618 3 View

Cultured human lymphocytes and virus stimulation?

Hi to all, I m started to grow lymphocytes Which cultures are positive to one sequences of the virus. Now, if I want to stimulate the production of the virus how can i do it? thanks for helping.

09 October 2016 4,030 1 View

PCR to detecte fragment of betaretrovirus sequence?

I m trying to amplified fragment of one betaretrovirus using primer which are designed on mouse sequence. I amplified something but when I extracted band on gel and sequenced that i don't read the...

09 October 2016 9,186 4 View

The best method to isolate and grow lymphocytes from fresh tessue?

Hello to all, i m starting to work with a culture of lymphocytes , but I want to know which is the best method for isolating these from tessue fresh surgery. Thanks for the help.

06 July 2016 1,461 4 View

Herceptin 150mg for Infusion is appropriate for Cell Culture?

03 April 2016 3,445 4 View

How can I confirm the mutation in PMS2 gene obtained through NGS?

03 April 2016 8,412 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View