FFPE RNA Seq Data?

Hi all, can anyone please help me to find dataset of Normal BRain FFPE trascriptomic data that i can use as a control i my experiment? Thank you for helping.

08 September 2018 111 0 View

3D spheroids and count?

Hi, I have a question about the count of 3D spheroids. How can I count the number of spheroids starting from the number of seeded cells? After 3 days colture I count spheroids that have 100 um...

02 March 2018 5,807 3 View

Study tumour haeterogenity

Hello,Can anyone suggest a bioinformatic approach to studying tumor heterogeneity in tumor samples? In other words, I performed exome analysis on my samples and now I want to build an evolutionary...

06 July 2017 7,989 1 View

How can evaluate the activity of inverseTrascriptase on a fresh sample?

Hi to all. someone knows or can suggest me a kit to evaluate the activities of inversetrascriptase the cell sample isolated from fresh tissue? Thanks so much

10 November 2016 4,595 4 View

Do you have a protocol to select Matrigel and prepare conditioned media to growing breast organoids?

I read some articles about organoids and their isolation from Frash fabric, but I have no idea and experience on how to select Matrigel and the average condition for growing and keeping them in...

09 October 2016 5,618 3 View

PCR to detecte fragment of betaretrovirus sequence?

I m trying to amplified fragment of one betaretrovirus using primer which are designed on mouse sequence. I amplified something but when I extracted band on gel and sequenced that i don't read the...

09 October 2016 9,186 4 View

The best method to isolate and grow lymphocytes from fresh tessue?

Hello to all, i m starting to work with a culture of lymphocytes , but I want to know which is the best method for isolating these from tessue fresh surgery. Thanks for the help.

06 July 2016 1,461 4 View

Herceptin 150mg for Infusion is appropriate for Cell Culture?

03 April 2016 3,445 4 View

How can I confirm the mutation in PMS2 gene obtained through NGS?

03 April 2016 8,412 10 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View