In PDA Beauveria and other entomopathogen suspected isolates grow very slowly when I cultured. Which media I may use or which salt/carbon source I may use to get rapid growth and spore rapidly. Need suggestions
Hi Shuvrah
In general fungi require longer time than bacteria to grow, I suggest to subculture a colony of what you call slow growth (24-48h old) in a fresh petri dish contained PDA (by the way if you have nutrient agar plate give it ago it may work), incubate at 25-28 C.
Hope this helps
Hello Dear
The most suitable mwdia for culturing of Beauveria is SDA. please see my publication about this subject. Beat Regards
Dear Shuvrah
In addition to PDA I recommend you to use : Rose Bengal agar or Carboxymethylcellulose liquid media and also YPD.
Hoping to be helpful
Marius
SDA medium is much better than PDA for entomopathogenic fungi like B. bassiana.
Dear Shuvrah
Although the right culture media such as SDA+Y may help to accelerate the growth of entomopathogenic fungi other factors such as temperature and light may affect the fungal growth. For example a complete darkness may be required for growth and sporulation of Beauveria isolates.
Mehran
Hello Dear , if you want a good growth you can use PDA , but if you want a good sporelation you should use MEA or PCA , while , if you want to isolate entomopathogenic fungi you should use SDA. with regards.
Hello, please see the following references:
A novel method to optimize culture conditions for biomass and sporulation of the entomopathogenic fungus Beauveria bassiana IBC1201 in Braz J Microbiol. 2011 Oct-Dec; 42(4): 1574–1584.
Mass production of entomopathogenic fungi Beauveria bassiana (Balsamo) by using agricultural products based on liquid- solid diphasic method for date palm pest control. In International Journal of Agriculture and Crop Sciences. Vol., 5 (19), 2337-2341, 2013
For agar based medium I routinely use SDAY (1/2 strength), or PDA, both with 1% yeast extract, for good sporulation. I find, in general, Beauveria 'prefers' SDAY, Metarhizium prefers PDAY (based on my working with ~500 strains of each). My experience with MEA (and ~ 50 Beauveria isolates) has been disappointing. OK mycelial growth but poor sporulation. For liquid media (e.g., for inoculating solid substrate) the simplest, for me, is 3% Dextrose, 1% yeast Extract, 1.5% (v/v) corn steep liquid and 0.2% Tween 80 (this is the Jenkins Prior Medium, modified by addition of corn steep). See my chapter in the latest Lacey Laboratory Methods Manual.
Regardless, it takes 5-7 days for Beauveria to sporulate well. Most researchers incubate plates for ~2 weeks.
Hello Stefan Jaronski, Jawad Abood Al-Janab and Yehya A. Salih thanks
I've found synthetic nutrient (Spezieller Nährstoffarmer) agar (SNA) induces good sporulation with minimal mycelia in Metarhizium spp., usually in ~10 days. I'm not sure if Beauveria behaves similarly.
SNA is used by Fusarium researchers to induce macroconidiation. And, since SNA is a defined medium, it should reduce somatic auxotrophic mutations.
Wort agar - for solid state fermentation
For submerged cultivation - medium on the base molasses, yeast or yeastrel, sodium nitrate, magnesium sulfate...
hello Dmitry Voitka yes this kind of information was much needed i.e. solid state and liquid culture. thanks a lot
Try it. Natural compounds are preffered for growth and sporulation of entomopathogenic fungi (as well as for antagonistic fungi).
Hello Shuvrah,
We use SNA medium and carrot, potato agar for Beauveria sporulation.
Dear Shuvrah,
I hope the following article may also useful reference for your question:
Senthamizhlselvan, et al., 2010. Growth, sporulation and biomass production of native entomopathogenic fungal isolates on a suitable medium. Journal of Biopesticides, 3(2): 466 – 469
Best regard
Jawad
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