What is the maximum number of personal articles that an author should cite in his or her new article?

It is a good practice for an author to cite some of his or her published work. However, this practice has been abused to gain citations.

21 December 2020 1,385 8 View

How does one decribe an HPLC chromatogram in a results section?

I happened to use HPLC in a project and the chromatograms are out. As a biologist, I don't really have the technical understanding to fully interpretate the results. I would like to know what to...

16 October 2020 7,006 5 View

I'm looking for a good pH 13 buffer recipe for hydrolysis experiment?

I did try phosphate using Na2HPO4.7H20 and NaOH but buffering capacity was very low especially upon dilution.

09 August 2020 9,692 4 View

I am faced with obtaining SVD and pseudo inverse of this (3x2) matrix A?

Please can someone help with the SVD of the matrix A and send me the pictures to my email? [email protected] Please note, I have tried the possible ways and I am still working on...

11 August 2019 8,468 3 View

Which single crystal growth technique is best for growing nice crystals from Co(III)-Hydrazone complexes?

I have synthesized Co(III)-Hydrazone complex and I want single crystals for SC-XRD measurement. I have tried varied crystal growth techniques: Vapour/solvent diffusion, slow evaporation, and...

04 February 2019 4,946 5 View

Can anyone recommend a Species Distribution Modelling short training / course for me?

I want to get further training in SDM to be able to build on the knowledge I have in order to do quality scientific research.

15 January 2019 7,896 5 View

Doubly periodic conditions (two-directions) for turbulent rectangular channel flow using LES in ANSYS Fluent?

I need a periodic BC where mass flow rate is specified to model fully developed flow, and another periodic BC with no pressure drop. However, once I define the mass flow rate for one periodic BC,...

18 November 2018 4,845 7 View

I'm planning a glucose uptake assay.

I'm planning a glucose uptake assay with adult mesenchymal stem cells and possibly rat neural stem cells for my undergraduate thesis. i'd appreciate some recommendations for uptake kits and...

18 July 2017 2,222 1 View

In soil contaminant transport, what are other modes of transport apart from advection, sorption and degradation?

03 February 2017 1,444 4 View

How do I pool RNA samples for sequencing?

We have done a disease model mouse experiment, for which there were two treatments, let's call them A and B, yes/no. So we have 4 strata, and we have 9+/-1 samples per strata. We aim to look...

13 September 2015 7,901 12 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View