How to exclude direct effect of drugs to neurons in mouse parkinson's model?

26 October 2020 1,127 2 View

LC3I band detection?

Hi, I have some questions about the electrophoresis and transfer condition. In our lab, we used 15% gel to SDS-page and semi-dry transfer In electrophoresis, i migrated the stacking gel in 80 V...

14 October 2020 4,046 2 View

Why were different results of the same sample measured in AFM?

I want to ask about Atomic Force Microscopy(AFM) tricky(?) things, I measured the same sample 2 times, similar position in the AFM. The sample is thin film made by evaporating on Si wafer. It...

13 September 2020 5,682 12 View

How can i solve this problem, OPV active film getting hazy?

Hi, guys.. These days, I have some problem in OPV processing. To be more specific, the active film is OK right after spin casting. But while drying it, the film is getting hazy..... it is changed...

07 January 2020 7,673 4 View

How to make SnO2 thin film by SnCl2-2H2O?

26 November 2019 5,407 3 View

What size of gold nanoparticles should I synthesize?

13 July 2019 5,878 2 View

how high temperature pepper fruits can resist?

I hope to monitor how fungal conidia (colletotrichum acutatum) infect on dead pepper fruits. so, I dipped sliced fragments of a pepper fruit in boiling water for 30 seconds and inoculated fungal...

11 July 2019 6,618 3 View

How to remove sealing film(:Metlonix 1170-60) from glass?

27 May 2019 4,644 2 View

What mean the Open circuit voltage are plus or minus?

Hellow, I am using 3 electrode system in chronocoulometry But Whenever I tried to do it, I saw Voc changed which are plus or minus. First time, I thought that the connection between counter and...

19 March 2019 7,143 4 View

Is it appropriate to use DMSO:Corn oil (50:50) in mouse as IP?

Is it appropriate to use DMSO:Corn oil mixture (50:50) in mouse as IP? I heard corn oil can induce colitis as IP, but there are many researchers using corn oil as in vivo test. I wonder your...

03 December 2018 4,092 1 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Which test should I use in SPSS if I have two independent variables and one dependent variable that is measured using two tests?

I am researching the effect of gender and video game use on reaction times. To do this, I will have participants complete two reaction time tests (a simple test and a four choice test). I am a...

17 February 2021 9,343 4 View

No title

17 February 2021 4,217 3 View

How can I find the thermophysical properties of high temperature microencapsulated phase change materials (MPCM)?

Hi everyone. I am looking for a high temperature MPCM. More specifically, I need the thermo physical properties of a microencapsulated phase change material with the melting temperature between...

14 February 2021 3,606 2 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View