06 June 2017 3 6K Report

Hello

I Performed real time pcr for 2 blood samples (cDNA) and got the attached results.

1. Why i can't see the Plateau line?  

2. Is this result acceptable? 

3. is the melt curve correct? and indicates that the amplification was only for my specific amplicon? not primer dimer..... etc?

I didn't run a control or a standard curve, I just wanted to test the amplification of my cDNA

thanks

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