Which PCR primer to use? No gene of intetest?

Hello, I work on serum samples and I'm in a big delimma regarding which primers to choose since I don't have a gene of interest because my purpose is to screen and identify a specific gene.

11 December 2019 2,405 1 View

How to get a list of candidate RNAs?

Hello, I'm wondering how to get a list of microRNA or lncRNA or other types of RNA that is associated with a certain type of cancer such as breast cancer? I see in research paper they often put...

07 August 2019 8,313 5 View

How to identify long non coding RNA using RT-qpcr?

Hi, How RT-qRCR can help me to IDENTIFY a lncRNAs? if i have deferentially expressed profiles...

04 May 2019 1,259 2 View

How to choose cell line for research?

03 April 2019 6,434 2 View

Gene experssion difference

07 August 2017 7,668 22 View

QPCR result

05 June 2017 5,556 3 View

RNA extraction

05 June 2017 1,923 10 View

Good RNA absorbance but to bands on gel

hello I am extracting RNA from Blood (50ul) using promega Reliaprep RNA cell miniprep system I measure the absorbance at 260 and got 344ug/ml however when I run the sample on gel, not bands appear...

04 May 2017 1,052 6 View

Primers after designing?

I designed primers for a specific DNA sequence, what is the next step? how can i get the primers in lab? form a company?

05 June 2016 9,241 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View