Which PCR primer to use? No gene of intetest?

Hello, I work on serum samples and I'm in a big delimma regarding which primers to choose since I don't have a gene of interest because my purpose is to screen and identify a specific gene.

11 December 2019 2,405 1 View

How to get a list of candidate RNAs?

Hello, I'm wondering how to get a list of microRNA or lncRNA or other types of RNA that is associated with a certain type of cancer such as breast cancer? I see in research paper they often put...

07 August 2019 8,313 5 View

How to identify long non coding RNA using RT-qpcr?

Hi, How RT-qRCR can help me to IDENTIFY a lncRNAs? if i have deferentially expressed profiles...

04 May 2019 1,259 2 View

Gene experssion difference

07 August 2017 7,668 22 View

QPCR result

05 June 2017 5,556 3 View

RNA extraction

05 June 2017 1,923 10 View

FTA Cards

05 June 2017 956 1 View

Good RNA absorbance but to bands on gel

hello I am extracting RNA from Blood (50ul) using promega Reliaprep RNA cell miniprep system I measure the absorbance at 260 and got 344ug/ml however when I run the sample on gel, not bands appear...

04 May 2017 1,052 6 View

Primers after designing?

I designed primers for a specific DNA sequence, what is the next step? how can i get the primers in lab? form a company?

05 June 2016 9,241 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View