Which PCR primer to use? No gene of intetest?

Hello, I work on serum samples and I'm in a big delimma regarding which primers to choose since I don't have a gene of interest because my purpose is to screen and identify a specific gene.

11 December 2019 2,405 1 View

How to get a list of candidate RNAs?

Hello, I'm wondering how to get a list of microRNA or lncRNA or other types of RNA that is associated with a certain type of cancer such as breast cancer? I see in research paper they often put...

07 August 2019 8,313 5 View

How to identify long non coding RNA using RT-qpcr?

Hi, How RT-qRCR can help me to IDENTIFY a lncRNAs? if i have deferentially expressed profiles...

04 May 2019 1,259 2 View

How to choose cell line for research?

03 April 2019 6,434 2 View

Gene experssion difference

07 August 2017 7,668 22 View

QPCR result

05 June 2017 5,556 3 View

RNA extraction

05 June 2017 1,923 10 View

FTA Cards

05 June 2017 956 1 View

Good RNA absorbance but to bands on gel

hello I am extracting RNA from Blood (50ul) using promega Reliaprep RNA cell miniprep system I measure the absorbance at 260 and got 344ug/ml however when I run the sample on gel, not bands appear...

04 May 2017 1,052 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View