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Questions related from Nono Mohd
Hello, I work on serum samples and I'm in a big delimma regarding which primers to choose since I don't have a gene of interest because my purpose is to screen and identify a specific gene.
12 December 2019 2,502 1 View
Hello, I'm wondering how to get a list of microRNA or lncRNA or other types of RNA that is associated with a certain type of cancer such as breast cancer? I see in research paper they often put...
08 August 2019 8,383 5 View
Hi, How RT-qRCR can help me to IDENTIFY a lncRNAs? if i have deferentially expressed profiles...
05 May 2019 1,328 2 View
Which cell line to choose for research if I'm working with a specific oncogene?
04 April 2019 6,503 2 View
Hi I have 2 sets of primers A and B, both of them target the same biomarker. I compared the gene expression values and notice a difference, my question is it possible that the gene expression will...
08 August 2017 7,744 22 View
Hello I Performed real time pcr for 2 blood samples (cDNA) and got the attached results. 1. Why i can't see the Plateau line? 2. Is this result acceptable? 3. is the melt curve correct? and...
06 June 2017 5,632 3 View
hello, I am extracting RNA from Blood and saliva using Promega Reliaprep RNA cell miniprep system in the lysis step it says that this step is to lyse cultured cells from suspension or adherent...
06 June 2017 1,997 10 View
hello do Whatman® FTA® cards preserve RNA of a sample? for example, I am using Whatman® FTA® cards to collect Blood and saliva samples and kept them for 1 month at room temperature.. is RNA preserved?
06 June 2017 1,035 1 View
hello I am extracting RNA from Blood (50ul) using promega Reliaprep RNA cell miniprep system I measure the absorbance at 260 and got 344ug/ml however when I run the sample on gel, not bands appear...
05 May 2017 1,153 6 View
I designed primers for a specific DNA sequence, what is the next step? how can i get the primers in lab? form a company?
06 June 2016 9,317 1 View
