How much of a difference will 5% FBS and 10% FBS make in cellular responses of mouse BMDM's?

I was wondering if culturing mouse BMDM's in 5% FBS vs 10% FBS will have significant differences in cell responses such as gene expression, cytokine production, etc.

09 October 2018 7,150 3 View

Can anyone suggest a reliable method/kit/reagent to detect ROI and RNI in formalin fixed cells?

I need to detect ROI and RNI in mouse immune cells, preferably using flow cytometry. I have to fix the cells before running them on flow. I tried optimizing the CellRoX green for ROI, but...

03 April 2018 1,247 1 View

Thoughts on Cell Press Sneak Peek?

I recently came across Cell Press Sneak Peek. Is it a good idea to use this option while the paper is in the first round of peer review? What if the paper is rejected after the review. Will you be...

10 November 2017 539 3 View

Technique to compare the levels of different proteins in the same tissue?

Hi, Can anyone help in suggesting a method/s to compare the levels of three different proteins with each other (i.e., if one is comparing proteins A,B,C and needs to show that protein A is...

31 December 2016 4,677 3 View

What is the maximum seeding for a 6-well plate that one can use (for RAW 264.7 macrophages)?

I need to do a 24 hours infection experiment and want to know how many cells/well (maximum) I can seed in a 6-well plate, 12 hrs prior to experiment. Reason being that I don't want to handle a ton...

11 December 2016 3,173 2 View

What is the specific purpose of using CD63 in flow cytometry ?

Can any one explain the specific purpose of using CD63 in flow experiments. Is it used as a maker for internalization ? Thanks!

08 September 2016 170 1 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View