Krishna Chinta

7 Questions 5 Answers 0 Followers

Questions related from Krishna Chinta

How much of a difference will 5% FBS and 10% FBS make in cellular responses of mouse BMDM's?

I was wondering if culturing mouse BMDM's in 5% FBS vs 10% FBS will have significant differences in cell responses such as gene expression, cytokine production, etc.

10 October 2018 7,236 3 View

Can anyone suggest a reliable method/kit/reagent to detect ROI and RNI in formalin fixed cells?

I need to detect ROI and RNI in mouse immune cells, preferably using flow cytometry. I have to fix the cells before running them on flow. I tried optimizing the CellRoX green for ROI, but...

04 April 2018 1,293 1 View

Thoughts on Cell Press Sneak Peek?

I recently came across Cell Press Sneak Peek. Is it a good idea to use this option while the paper is in the first round of peer review? What if the paper is rejected after the review. Will you be...

11 November 2017 587 3 View

Technique to compare the levels of different proteins in the same tissue?

Hi, Can anyone help in suggesting a method/s to compare the levels of three different proteins with each other (i.e., if one is comparing proteins A,B,C and needs to show that protein A is...

01 January 2017 4,727 3 View

What is the maximum seeding for a 6-well plate that one can use (for RAW 264.7 macrophages)?

I need to do a 24 hours infection experiment and want to know how many cells/well (maximum) I can seed in a 6-well plate, 12 hrs prior to experiment. Reason being that I don't want to handle a ton...

12 December 2016 3,225 2 View

What is the specific purpose of using CD63 in flow cytometry ?

Can any one explain the specific purpose of using CD63 in flow experiments. Is it used as a maker for internalization ? Thanks!

09 September 2016 224 1 View

Protocol for the detection of ROS and RNS in mouse lung cell isolates using flow cytometry?

Can anyone share a protocol to detect ROS and RNS in the immune cells isolated from mouse lungs, using flow cytometry. NOTE: Fixing of these cells before running them on the flow-cytometer is...

08 August 2016 6,056 1 View