How much of a difference will 5% FBS and 10% FBS make in cellular responses of mouse BMDM's?

I was wondering if culturing mouse BMDM's in 5% FBS vs 10% FBS will have significant differences in cell responses such as gene expression, cytokine production, etc.

09 October 2018 7,150 3 View

Can anyone suggest a reliable method/kit/reagent to detect ROI and RNI in formalin fixed cells?

I need to detect ROI and RNI in mouse immune cells, preferably using flow cytometry. I have to fix the cells before running them on flow. I tried optimizing the CellRoX green for ROI, but...

03 April 2018 1,247 1 View

Thoughts on Cell Press Sneak Peek?

I recently came across Cell Press Sneak Peek. Is it a good idea to use this option while the paper is in the first round of peer review? What if the paper is rejected after the review. Will you be...

10 November 2017 539 3 View

Technique to compare the levels of different proteins in the same tissue?

Hi, Can anyone help in suggesting a method/s to compare the levels of three different proteins with each other (i.e., if one is comparing proteins A,B,C and needs to show that protein A is...

31 December 2016 4,677 3 View

What is the maximum seeding for a 6-well plate that one can use (for RAW 264.7 macrophages)?

I need to do a 24 hours infection experiment and want to know how many cells/well (maximum) I can seed in a 6-well plate, 12 hrs prior to experiment. Reason being that I don't want to handle a ton...

11 December 2016 3,173 2 View

Protocol for the detection of ROS and RNS in mouse lung cell isolates using flow cytometry?

Can anyone share a protocol to detect ROS and RNS in the immune cells isolated from mouse lungs, using flow cytometry. NOTE: Fixing of these cells before running them on the flow-cytometer is...

07 August 2016 6,010 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View