I have PEG conjugated antibody in pH 10 borate buffer. I have to run SDS PAGE to determine MW. In this case prior running the gel do I need to do buffer change of the sample? Like borate buffer to PBS?
That depends on the concentration of the buffer. The more ions are present in your sample, the more they will affect electrophoresis. 10 or even 20 mM should be fine, otherwise I'd do a chloroform/methanol precipitation (doi:10.1016/0003-2697(84)90782-6) to quickly remove interfering substances.
Agree with Engelberth. If your borate buffer is not of a high concentration, you could use the smallest possible volume of your sample and mix it with a larger volume of highly concentrated SDS-sample buffer, to achieve an adequate final pH and run the sample successfully.
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