Can anyone suggest to me the tool for gene annotation and clustering of microarray data?

31 December 2014 5,551 3 View

Can anyone help me with PI staining of the cell line?

I used PI stain for Jurkat cell line, in this control (normal) cells also give fluorescence. Can anyone suggest what can be the problem?

03 April 2014 3,521 3 View

Does anyone (in India) have Jurkat E6.1 and SH-SY5Y cell lines?

As NCCS Pune has stopped to supply these cell lines, I am not able to get them.

01 February 2014 2,841 2 View

Microarray raw data submission?

What is the procedure to submit raw data of microarrays? In which microarray database I should submit my raw data and is it compulsory to submit the data before communicate the article?

31 December 2013 2,307 6 View

Anyone suggest me how to lyophilize the 2D-Protein gel plugs for the MALDI-TOF analysis?

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11 December 2013 9,854 1 View

Can anyone suggest a suitable cell line for a study of the effects of neurotoxic agents on human neuroblastoma?

We have an IMR-32 cell line. Can anyone suggest whether this is a good cell line for the above study?

09 October 2013 9,083 3 View

How to do mitochondrial membrane potential?

I want to do mitochondrial membrane potential of human cell line. can anyone tell me the procedure of the assay? *We don't have flowcytometer in our lab.

08 September 2013 5,027 7 View

Hoechst staining of HL-60 cell line?

I would like to perform fluorescence microscopy on a HL-60 cell line using Hoechst. How to stain the suspension culture with this stain? Can anybody send me a detailed protocol?

08 September 2013 1,707 2 View

How to lyse cell culture for the analysis of ROS/RNS using kit ??

I am planing to use a kit [(from cell biolabs (cat # STA-347) ] for the analysis of total ROS. In this kit it is given to sonicate or homogenize the cells but we don't have sonicator in our lab....

05 June 2013 1,933 4 View

Micronucleus assay on HL-60 cell line?

I am doing this assay on HL-60 cell line and every time I am getting broken cell membrane hence I am not able to see micronuclei. Does anyone have experience with this assay? Please send me any...

05 June 2013 9,850 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View