Nicolas J Pillon

8 Questions 25 Answers 0 Followers

Questions related from Nicolas J Pillon

When preparing the figures for a paper, how should we write the name of the proteins and their phosphorylation state?

I think the rule is that the protein should be capitalized, but in the case of MAPK, people use the non-capitalized form (p38 and p42 for instance). Is that because it is a subunit? Related to...

11 November 2012 6,910 6 View

Why do most people think that melting of the arctic ice will not change the level of oceans?

I've heard so many times the argument of a melting iceberg floating in fresh water that will not change the volume of water when melting. But this is of course not valid for an iceberg floating in...

08 August 2012 1,537 7 View

Is it possible to extract RNA from 96-well plates for qPCR?

I need a method to measure gene expression by qPCR in very small samples, ideally cells grown in 96-well plates. What method should I use? Any suggestion will be welcome!

07 July 2012 6,859 8 View

What lipopolysaccharides should be used to mimic inflammation in obesity?

LPS can come from E. Coli, Salmonella and many other bacteria. Which one should be a good control to induce inflammation in animals and macrophages in culture?

01 January 1970 3,032 11 View

How to get rid of proteins in supernatant from cells?

I want to remove/inactivate all proteins and peptides from my solution. I was thinking of using proteinase K to cut them all. Any idea of what concentration/time I should use, or any other method...

01 January 1970 6,017 6 View

How does one estimate the differentiation of THP-1 monocytes into macrophages?

Standard procedure to differentiate THP-1 cell is treatment with PMA and selection of adherent cells. Is there any specific markers that can be used to confirm the differentiation process, or that...

01 January 1970 803 10 View

How can I measure nucleotides in culture media?

Is there an easy way to measure ATP, ADP, UTP and UDP in cell culture media? What would be the sensitivity of these assays?

01 January 1970 5,639 2 View

What connexins/pannexins are expressed in skeletal muscle?

There are several sub-types of pannexin and connexin channels that are expressed either during embryogenesis or in different tissues in adults. From your experience, does anybody knows which ones...

01 January 1970 2,729 5 View