I have cultured cyanobionts, isolated from lichens. I isolate DNA. Now for molecular studies i want to amplify DNA. so please suggest me best primer set for cyanobionts like 16S.
Which manufactures/MNC'S EXOSAP needed 10 times dilution before use?
04 May 2019 5,688 1 View
Hello folks, I have amplified 4 cultured filamentous cyanobacteria isolated from lichens using Cyano 106F and Cyano 781 R primers. PCR band was very good. Then i go for sequencing using same...
04 May 2019 2,377 3 View
How to prepare a stock solution of lyophilized primer. Which one is good for dilution TE and water?
08 September 2018 6,756 4 View
I am amplified DNA of algae by using ITS universal primer. In some PCR reaction i got double band, how can i reduce it?
07 August 2018 7,119 15 View
I want to stain nucleus of green algae by DAPI or Hoechst 33342 by using confocal microsopy. if anyone have appropriate protocol please send to me?
07 August 2018 7,454 6 View
I want to microscopic studies of cultured unicellular algal cells. I want to know which dye is most widely used for nucleus staining for algae.
03 April 2018 920 8 View
I am isolated DNA from some folliose lichens, then purified in ethanol at 13000 rpm, after 15 minute drying, pellet doesn't dissolved in elution buffer. I am also using warm water and ( 65 C)...
03 April 2018 2,561 5 View
I am using 3N BBM broth media in a 250 ml flask for unicellular green algae cultivation. Which condition is very good for faster algal growth? How much days will take for good growth?
03 April 2018 1,361 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...
08 August 2024 6,134 12 View
I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...
08 August 2024 4,812 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View