Khem Chand Saini

9 Questions 21 Answers 0 Followers

Questions related from Khem Chand Saini

How to Sequence cultured Cyanobacteria (most probably Nostoc spp.) Isolated from lichens?

Hello folks, I have amplified 4 cultured filamentous cyanobacteria isolated from lichens using Cyano 106F and Cyano 781 R primers. PCR band was very good. Then i go for sequencing using same...

05 May 2019 2,336 3 View

Does Exosap needed 10 times dilution for cleaning for PCR product for sequencing?

Which manufactures/MNC'S EXOSAP needed 10 times dilution before use?

05 May 2019 5,651 1 View

How to prepare a stock solution of lyophilized primer. Which one is good for dilution TE and water?

09 September 2018 6,717 4 View

How can reduce multiple band/double band problem in PCR amplification?

I am amplified DNA of algae by using ITS universal primer. In some PCR reaction i got double band, how can i reduce it?

08 August 2018 7,085 15 View

I want to stain nucleus of green algae by DAPI or Hoechst 33342 for confocal microsopy, which one is giving best results?

I want to stain nucleus of green algae by DAPI or Hoechst 33342 by using confocal microsopy. if anyone have appropriate protocol please send to me?

08 August 2018 7,425 6 View

Please suggest me primers for cyanobionts ( cyanobacteria) isolated from the lichens?

I have cultured cyanobionts, isolated from lichens. I isolate DNA. Now for molecular studies i want to amplify DNA. so please suggest me best primer set for cyanobionts like 16S.

07 July 2018 9,685 0 View

I am isolated DNA from folliose lichens, but pellet not dissolved in elution buffer?

I am isolated DNA from some folliose lichens, then purified in ethanol at 13000 rpm, after 15 minute drying, pellet doesn't dissolved in elution buffer. I am also using warm water and ( 65 C)...

04 April 2018 2,529 5 View

Which dye is most suitable for nucleus staining of unicellular algae?

I want to microscopic studies of cultured unicellular algal cells. I want to know which dye is most widely used for nucleus staining for algae.

04 April 2018 891 8 View

For unicellular algal broth culture what is optimum condition like temprature, Light and Shaking speed (RPM?) for faster growth?

I am using 3N BBM broth media in a 250 ml flask for unicellular green algae cultivation. Which condition is very good for faster algal growth? How much days will take for good growth?

04 April 2018 1,327 3 View