Which manufactures/MNC'S EXOSAP needed 10 times dilution before use?
After the cleaning with ExoSap, you need to diluted your product 1:10 and use for the PCR with BigDye 2 ul.
Hello folks, I have amplified 4 cultured filamentous cyanobacteria isolated from lichens using Cyano 106F and Cyano 781 R primers. PCR band was very good. Then i go for sequencing using same...
04 May 2019 2,377 3 View
How to prepare a stock solution of lyophilized primer. Which one is good for dilution TE and water?
08 September 2018 6,756 4 View
I am amplified DNA of algae by using ITS universal primer. In some PCR reaction i got double band, how can i reduce it?
07 August 2018 7,119 15 View
I want to stain nucleus of green algae by DAPI or Hoechst 33342 by using confocal microsopy. if anyone have appropriate protocol please send to me?
07 August 2018 7,454 6 View
I have cultured cyanobionts, isolated from lichens. I isolate DNA. Now for molecular studies i want to amplify DNA. so please suggest me best primer set for cyanobionts like 16S.
06 July 2018 9,714 0 View
I want to microscopic studies of cultured unicellular algal cells. I want to know which dye is most widely used for nucleus staining for algae.
03 April 2018 920 8 View
I am isolated DNA from some folliose lichens, then purified in ethanol at 13000 rpm, after 15 minute drying, pellet doesn't dissolved in elution buffer. I am also using warm water and ( 65 C)...
03 April 2018 2,561 5 View
I am using 3N BBM broth media in a 250 ml flask for unicellular green algae cultivation. Which condition is very good for faster algal growth? How much days will take for good growth?
03 April 2018 1,361 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View