I want to microscopic studies of cultured unicellular algal cells. I want to know which dye is most widely used for nucleus staining for algae.
Have you tried DAPI? Check out the attached publication:
"To improve the effectiveness of DAPI staining, we useda microwave oven heating method that was originally developed for the retrieval of antigens from paraffin-embedded tissue in immunohistochemical staining (Shi et al. 1991). The addition of this microwave oven treatmentto the DAPI staining method effectively eliminated the effect of the thick cell walls, and allowed DAPI to stain the cell nucleus." (referring to algae)
Dear Mr. Saini,
In addition to DAPI, acridine orange is also used to stain nuclei of coccoidal algae . Lipophilic dyes such as Nile Red (Nile Blue Oxazone), BODIPY ( 4,4-difluoro-4- bora-3a,4a-diaza-s-indacene ) or DiO ( 3,30 -dioctadecyloxacarbocyanine perchlorate) are also used to stain algal cells
Hope this helps you.
Regards,
Dr. Abhishek Mukherjee
Hi,
It's depend on the nature of the sample (If was fixed or not)
I recommend the use of hoechst 33342 or Sybr GreenI.
Article Analysis of phytoplankton by flow cytometry
Article Enumeration and Cell Cycle Analysis of Natural Populations o...
For live cells? I recommend Hoechst 33342 (blue) or SYTO 9 (green).
Jason A Kilgore Does Hoechst 33342 is suitable for staining of nucleus algal cells? if yes please send me protocol?
Khem Chand Saini Yes, Hoechst has been used for labeling algae. Here's an example paper. Perhaps they have some protocol information: https://link.springer.com/article/10.1007%2Fs00284-008-9150-8
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