Anita Balazs Hey, I want to stain nucleus of algae with Hoechst 33342 but problem in permeability, i think coz of cell wall. So can u help what can i do?
Khem Chand Saini you can use DMSO, for example, to help the dye enter into the cell. You could test different DMSO concentrations and incubation times.
I am used to use the "ProLong Gold Antifade Mountant with DAPI" to prepare slides of Chlamydomonas reinhardtii and it has been working in order to see the nuclei in fluorescence microscopy.