Does Exosap needed 10 times dilution for cleaning for PCR product for sequencing?

Which manufactures/MNC'S EXOSAP needed 10 times dilution before use?

04 May 2019 5,558 1 View

How to Sequence cultured Cyanobacteria (most probably Nostoc spp.) Isolated from lichens?

Hello folks, I have amplified 4 cultured filamentous cyanobacteria isolated from lichens using Cyano 106F and Cyano 781 R primers. PCR band was very good. Then i go for sequencing using same...

04 May 2019 2,222 3 View

How to prepare a stock solution of lyophilized primer. Which one is good for dilution TE and water?

How to prepare a stock solution of lyophilized primer. Which one is good for dilution TE and water?

08 September 2018 6,636 4 View

I want to stain nucleus of green algae by DAPI or Hoechst 33342 for confocal microsopy, which one is giving best results?

I want to stain nucleus of green algae by DAPI or Hoechst 33342 by using confocal microsopy. if anyone have appropriate protocol please send to me?

07 August 2018 7,365 6 View

How can reduce multiple band/double band problem in PCR amplification?

I am amplified DNA of algae by using ITS universal primer. In some PCR reaction i got double band, how can i reduce it?

07 August 2018 7,011 15 View

Please suggest me primers for cyanobionts ( cyanobacteria) isolated from the lichens?

I have cultured cyanobionts, isolated from lichens. I isolate DNA. Now for molecular studies i want to amplify DNA. so please suggest me best primer set for cyanobionts like 16S.

06 July 2018 9,627 0 View

Which dye is most suitable for nucleus staining of unicellular algae?

I want to microscopic studies of cultured unicellular algal cells. I want to know which dye is most widely used for nucleus staining for algae.

03 April 2018 831 8 View

I am isolated DNA from folliose lichens, but pellet not dissolved in elution buffer?

I am isolated DNA from some folliose lichens, then purified in ethanol at 13000 rpm, after 15 minute drying, pellet doesn't dissolved in elution buffer. I am also using warm water and ( 65 C)...

03 April 2018 2,458 5 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

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02 March 2021 3,060 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

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28 February 2021 9,936 2 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View