Hello folks, I have amplified 4 cultured filamentous cyanobacteria isolated from lichens using Cyano 106F and Cyano 781 R primers. PCR band was very good. Then i go for sequencing using same primers. I got sequence results but when i go for Blast search its showing match with Uncultured bacterial gene. What will i do? what u suggest, which primers combination will i used or anything else?