Hi, I want to some miRNA study like miRNA profiling and microarray studies for So, I have to extract miRNA from cells like macrophage. please suggest a method with reference for that. Thank you..
Circulating MicroRNAs: Methods and Protocols, Methods in Molecular Biology, vol. 1024
The most commonly used method is probably TRIzol (Ambion) however a column-based kit (such as Qiagen's miRNeasy kit) may decrease variability in your results. PMIDs for a couple of PubMed publications you may find interesting:27321975, 24885883.
Hi Sandeep,
We use mirVana Kit to get best results.
Warm regards,
Venk
Thank you all :)
I have gone through literature and found that miRvana is most commonly used ( as suggested by Venkatesh) but TRIzol and Tri reagent are also better especially in stored samples.
Hi Sandeep
I have tried Tri reagent and kit and I agree with Venkatesh. I prefer to use a kit (mirVana is very good) rather then Tri reagents because they may lead to miRNA loss. This can be harmfull to profiling and microarray studies. Besides that the quality of the miRNA is higher using the kit.
hi .. i've used norgen to extract miRNA frem serum and plasma
good luck
Hi Sandeep
I also prefer column-based kits than TRIzol. If you are worried about the yield, you can find a slightly modified column-based extraction from McAlexander et al. 2013 and Burgos et al. 2013. One with the addition of glycogen as a carrier and the other basically with an extra re-extraction step, both worked for me
Hi
I use Qiagen miRNeasy micro kits. I tried mirVana but got higher amounts from the Qiagen kit and it's really easy.
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