11 Questions 23 Answers 0 Followers
Questions related from Sandeep Rai Kaushik
Hi, I am using serum samples for western blotting. I have used different antibodies (for different target proteins) and most of them are monoclonal. I am getting unusual results like a band around...
12 December 2019 4,216 6 View
Hi, I have 3 cell lines viz. J774A.1, RAW264.7 and A549. I want to do a GCMS analysis for some experiment. Please suggest a suitable method for analyte extraction. Thank you
12 December 2018 7,901 5 View
I am planning to use ELISA from Human SErum samples. My aim is quantitative analysis. I have few questions: 1. DO we need to standardise the protocol for our samples or can we directly use the...
10 October 2018 4,093 7 View
I am curious to know if we can use the ICP grade standards (https://www.sigmaaldrich.com/catalog/product/mm/109492?lang=en®ion=IN) for ICP-MS application...
05 May 2018 6,173 4 View
Hi, I am using A549 cells. I have taken a cryovial from -80 to revive the cells. I have revived at 37 for around 2 min and then gave two media wash. then seeded all the cells in a T-25 flask...
10 October 2016 5,872 4 View
Hi, I have to use DPPH assay for antioxidant activity of a compound from plant origin. I am using Ascorbic acid as standard. I just want to know is it necessary to use same solvent for DPPH and...
10 October 2016 171 9 View
I have run an SDS PAGE and after Silver-staining, I am getting extra thin lanes (Vertical) bifurcated from main lane. What could be the possible expalnations for these thin lanes??
08 August 2016 624 9 View
Hi, I want to some miRNA study like miRNA profiling and microarray studies for So, I have to extract miRNA from cells like macrophage. please suggest a method with reference for that. Thank you..
07 July 2016 2,354 8 View
I have extracted the Genomic DNA from Candida albicans. Instead of a High size band i got a single very bright very below in gel. What it can be and why it happened?Note: I did not use RNAse...
05 May 2016 1,879 11 View
I have many papers on serum zinc estimation by using Bromo PAPS and Nittro PAPS. I just want to know which of these two give better results. If anybody is working with or had any experience,...
04 April 2016 5,209 0 View
I'm doing iron quantification using Ferrozine assay but very low or negligible absorbance in serum samples. I am getting very good absorbance in standards. I am using Ascorbic acid as reducing...
03 March 2016 7,314 2 View