What is common protein of about 50kDa in serum samples?

Hi, I am using serum samples for western blotting. I have used different antibodies (for different target proteins) and most of them are monoclonal. I am getting unusual results like a band around...

11 December 2019 4,158 6 View

How to extract sample for GC/MS from cell lines?

Hi, I have 3 cell lines viz. J774A.1, RAW264.7 and A549. I want to do a GCMS analysis for some experiment. Please suggest a suitable method for analyte extraction. Thank you

11 December 2018 7,835 5 View

Sandwich ELISA vs Competitive ELISA?

I am planning to use ELISA from Human SErum samples. My aim is quantitative analysis. I have few questions: 1. DO we need to standardise the protocol for our samples or can we directly use the...

09 October 2018 3,988 7 View

Can we use icp standards for icp ms?

I am curious to know if we can use the ICP grade standards (https://www.sigmaaldrich.com/catalog/product/mm/109492?lang=en®ion=IN) for ICP-MS application...

04 May 2018 6,088 4 View

Why my cells did not get adhered?

Hi, I am using A549 cells. I have taken a cryovial from -80 to revive the cells. I have revived at 37 for around 2 min and then gave two media wash. then seeded all the cells in a T-25 flask...

09 October 2016 5,797 4 View

Solvent for DPPH and Antioxidant should be same or can be different?

Hi, I have to use DPPH assay for antioxidant activity of a compound from plant origin. I am using Ascorbic acid as standard. I just want to know is it necessary to use same solvent for DPPH and...

09 October 2016 113 9 View

Why lanes in SDS PAGE are bifurcating in an extra thin lane?

07 August 2016 564 9 View

Please suggest best method for "miRNA" extraction?

Hi, I want to some miRNA study like miRNA profiling and microarray studies for So, I have to extract miRNA from cells like macrophage. please suggest a method with reference for that. Thank you..

06 July 2016 2,298 8 View

Genomic DNA on Agarose gel?

04 May 2016 1,816 11 View

What should I do to get better absorbance in my serum samples by Ferrozine assay??

I'm doing iron quantification using Ferrozine assay but very low or negligible absorbance in serum samples. I am getting very good absorbance in standards. I am using Ascorbic acid as reducing...

02 March 2016 7,260 2 View

No title

02 March 2021 932 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View