I have many papers on serum zinc estimation by using Bromo PAPS and Nittro PAPS. I just want to know which of these two give better results. If anybody is working with or had any experience, please suggest..
Thank you..
Hi, I am using serum samples for western blotting. I have used different antibodies (for different target proteins) and most of them are monoclonal. I am getting unusual results like a band around...
11 December 2019 4,248 6 View
Hi, I have 3 cell lines viz. J774A.1, RAW264.7 and A549. I want to do a GCMS analysis for some experiment. Please suggest a suitable method for analyte extraction. Thank you
11 December 2018 7,919 5 View
I am planning to use ELISA from Human SErum samples. My aim is quantitative analysis. I have few questions: 1. DO we need to standardise the protocol for our samples or can we directly use the...
09 October 2018 4,123 7 View
I am curious to know if we can use the ICP grade standards (https://www.sigmaaldrich.com/catalog/product/mm/109492?lang=en®ion=IN) for ICP-MS application...
04 May 2018 6,206 4 View
Hi, I am using A549 cells. I have taken a cryovial from -80 to revive the cells. I have revived at 37 for around 2 min and then gave two media wash. then seeded all the cells in a T-25 flask...
09 October 2016 5,890 4 View
Hi, I have to use DPPH assay for antioxidant activity of a compound from plant origin. I am using Ascorbic acid as standard. I just want to know is it necessary to use same solvent for DPPH and...
09 October 2016 198 9 View
I have run an SDS PAGE and after Silver-staining, I am getting extra thin lanes (Vertical) bifurcated from main lane. What could be the possible expalnations for these thin lanes??
07 August 2016 646 9 View
Hi, I want to some miRNA study like miRNA profiling and microarray studies for So, I have to extract miRNA from cells like macrophage. please suggest a method with reference for that. Thank you..
06 July 2016 2,377 8 View
I have extracted the Genomic DNA from Candida albicans. Instead of a High size band i got a single very bright very below in gel. What it can be and why it happened?Note: I did not use RNAse...
04 May 2016 1,906 11 View
I'm doing iron quantification using Ferrozine assay but very low or negligible absorbance in serum samples. I am getting very good absorbance in standards. I am using Ascorbic acid as reducing...
02 March 2016 7,333 2 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
how to get links for copyrights for papers?
06 August 2024 7,410 1 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?
04 August 2024 1,537 1 View
need to open an account to upload my published papers
01 August 2024 9,255 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...
31 July 2024 7,259 1 View
I've been using ResearchGate for a while now and have found many useful research papers that are beneficial for my work. However, I've noticed that many authors do not respond to my messages....
31 July 2024 7,771 5 View
I have not addede any paper yet but it has selected 3 papers which are not mine in my account. i want to delete that information. please help me
30 July 2024 3,743 0 View