Zaynab Saad

12 Questions 56 Answers 0 Followers

Questions related from Zaynab Saad

Why different protein activity from multiple codons for the same amino acid???

hii all i'm asking why multiple codons code for the same amino acid but different protein activity ? please attach papers or reviews thank you all zaynab

12 December 2018 2,799 5 View

"kit of pyruvate concentration" for serum samples are good or suitable for cell line suspension??

Have a nice day colleague i'm going to determine pyruvate concentration from cell line suspension (before and after treatment) for different duration times using Elabscience pyruvate assay kit...

10 October 2018 1,915 3 View

Is there protocol for restraining slids of immunohistchemistry?

Have a nice day every one I’ve prepared slides for immunohistochemistery finished staining with DAP and Heamatoxyline and put the covers lip but unfortunately the staining was poor I want...

05 May 2018 493 2 View

Is leaving your cell in TRİZOL safe ????

Hello I’m about to extract total RNA from cell line using TRIZOL ... is it safe to scrab first cell and put the trizol then freezing and complet the rest of proceduer next day .. or scrab cell...

04 April 2018 3,254 4 View

Iraq cancer registry 1990-2000??

have a nice day i'm very interested to study the statistic and gradual increase of cancer incidence in iraq specially between 1999-2000 year i search and couldn't find something i can depend just...

01 January 2018 6,503 1 View

How can i prepare skimmed milk?

Hi ... I'm about to do western blotting. ... and i want to block using skimmed milk How can i prepare it ? Or can i use powder one ? Thanks

04 April 2016 3,530 11 View

MicroRNA from mesenchymal stem cell?

Hi I want to extract microRNA from mesenchymal stem cell After collecting MSC by magnatic beads.... which best kit can i utilise to get pure with good concentration of MIRNA ? Can i use norgen...

04 April 2016 3,534 3 View

I have an extra band ... why?

good day i have dissociation curve of b- actin gene of two cell line (control and treated) one of the curve perfect while the other has a good curve with extra small curve after that. why does...

03 March 2016 4,162 3 View

Any protocol for protein extraction?

Hi ... I've scraped cancer cell line without throughout the media ... all the suspension transfered to a tube and stored at -80 C. How can i extract protein from this one please any ideas or...

03 March 2016 6,331 7 View

Is their any differences between ECL western blotting apparatus and gel band fluorescent detection apparatus?

Hi ... in lab. I have band ge detectionl fluorescent apparatus and I'm undergoing to do western blotting using enhanced chemiluminescence (ECL) My question is ... can i determine the bands on the...

01 January 2016 2,910 2 View

Why are there no microRNAs found on Y chromosome?

While reading a lot of articles about biogenesis and importance of microRNAs in diagnosis of diseases and cancer and the ability of using microRNAs in treatment ... in this time i faced that there...

12 December 2015 2,706 5 View

Is it right to use GAPDH TaqMan as an endogenous control to calculate expression of microRNA?

hi is it right to use GAPDH TaqMan as a reference gene instead of using miR-16 or U6 to determine the expression of target miRNAs such as miR-21 ? thanks

11 November 2015 4,148 5 View