How do I further isolate uncoloured fractions that show peaks on HPLC?

Hello Colleagues, I have several fractions (Hex:EtAc). In one small fraction (about 2mls), I got 6 peaks on HPLC. What do I need to do further or how do I need to separate these compounds,...

09 October 2018 1,547 8 View

How can I prepare a preparative TLC plate (PTLC) using silica gel? or where can I buy already prepared PTLC plates?

Dear Colleagues, Please help. How can I prepare a preparative TLC plate (PTLC) using silica gel? or where can I buy already prepared PTLC plates (Close to Malawi)?

08 September 2018 3,606 3 View

At what point does sequential solvent extraction make more sense? At crude extraction or fractionation?

Dear colleagues, At what point does sequential solvent extraction make more sense? At crude extraction or fractionation stage? One other thought would be to do crude extraction using MeOH and do...

07 August 2018 3,616 4 View

What is the best way of working with extremely busy co-authors in a manuscript?

Dear researchers, Sometimes we have to work with our highly respected co-authors that are extremely too busy to give time to our manuscriots, but for some important reasons, they have to be on the...

05 June 2018 9,949 22 View

What are the most common non-material addiction drugs you know?

What are the most common non-material addiction drugs you know? - Please share if you know any conventional drugs on the market

05 June 2018 2,728 0 View

What is you experience/knowledge about crossing borders with research plant sample extracts?

What is your experience/knowledge about crossing borders with research plant sample extracts? - Any challenges? - Noteworthy details? Please share Thank you and kind regards

05 June 2018 2,226 0 View

Which is better to extract: From fresh or dried plant samples?

which is better to extract from fresh or dried plant samples?

05 June 2018 529 0 View

Is it worthy spending resources on researching orally and intravenously effective drugs from polar compounds?

Dear researchers, I understand that we can get drugs from polar compounds, but these are usually hardly bioavailable. This means the drug has to keep being modified to improve it's bioavailability...

05 June 2018 9,842 6 View

Do you know any medicinal properties of chlorophyll?

Do you know any medicinal properties of chlorophyll? Please share your ideas

05 June 2018 6,720 11 View

Please share any links or documents on specifications for drug tablets and syrup?

Please share any links or documents on specifications for drug tablets and syrup - Physical parameters as well as chemical parameters, I will appreciate

04 May 2018 6,540 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View