Please, any lab in Tanzania that is currently doing cytotoxicity tests on cancerous cell lines?
Kindly let me know
Regards
Hello Colleagues, I have several fractions (Hex:EtAc). In one small fraction (about 2mls), I got 6 peaks on HPLC. What do I need to do further or how do I need to separate these compounds,...
09 October 2018 1,711 8 View
Dear Colleagues, Please help. How can I prepare a preparative TLC plate (PTLC) using silica gel? or where can I buy already prepared PTLC plates (Close to Malawi)?
08 September 2018 3,845 3 View
Dear colleagues, At what point does sequential solvent extraction make more sense? At crude extraction or fractionation stage? One other thought would be to do crude extraction using MeOH and do...
07 August 2018 3,801 4 View
Dear researchers, Sometimes we have to work with our highly respected co-authors that are extremely too busy to give time to our manuscriots, but for some important reasons, they have to be on the...
05 June 2018 10,104 22 View
What are the most common non-material addiction drugs you know? - Please share if you know any conventional drugs on the market
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which is better to extract from fresh or dried plant samples?
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05 June 2018 2,371 0 View
Dear researchers, I understand that we can get drugs from polar compounds, but these are usually hardly bioavailable. This means the drug has to keep being modified to improve it's bioavailability...
05 June 2018 9,995 6 View
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05 June 2018 6,956 11 View
Please share any links or documents on specifications for drug tablets and syrup - Physical parameters as well as chemical parameters, I will appreciate
04 May 2018 6,690 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
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I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
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Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View