I have purified a protein using a protocol provided in a reasearch paper but this protein unable to bind to RNA (emsa) used as a positive control.Why?

The protein purified by them is showing binding to the RNA. I have used exactly same lysis, washing and elution buffer. I have got purified very good purified protein.

03 April 2018 9,634 10 View

Any online software or database available that provides information about loss of heterozygosity of a gene in a cell line?

If one is interested to know whether a gene resides in loss of heterozygosity region of a cell line, what are the ways he/she can resolve it? Is there any database or online bioinformatic tool is...

10 November 2016 1,271 1 View

How to use the NCBI GEO analysis/data-sets to find out expression profile of a particular gene?

In research articles people often show meta analysis of a gene using NCBI GEO data sets or analysis, i want to know the procedure by which i can derive the information for a particular gene?

01 February 2015 2,395 3 View

What is "Application Note" in a journal, does it have same value as research article publication?

I want to understand the importance of :Application note" section of a journal, Is it same as publishing a research article and have the same value (in terms of Impact factor of that journal) as...

31 December 2014 6,059 2 View

Ethical and technical issue

07 August 2014 7,114 1 View

Can we consider HEK 293T as a normal cell line?

If one is performing some experiments with cancerous cell lines, will it be a correct strategy to use HEK 293T as a normal cell line as a control?

02 March 2014 1,677 13 View

Concentration of L-Lysine and L-Arginine in normal RPMI and DMEM?

Can anybody inform me the concentration of L-Lysine and L-Arginine in normal RPMI and DMEM media?

01 February 2014 10,015 7 View

How to calculate real-time PCR data?

I am confused about how to calculate the fold change of transcript level by real time PCR, suppose in two conditions: control : GAPDH - 25 gene X- 18 Test : GAPDH - 23 gene X - 20 What will be...

31 December 2013 1,167 14 View

What are the ways a person can test the drug resistance for a particular cell line for a particular drug?

What are the experimental methods one can perform to find out whether a cell line is really resistance to a certain drug or not?

10 November 2013 1,163 3 View

How to remove the stains of developer from cloths?

Its a silly question.. one of my colleague asked me this question, but I couldn't answer so I am asking it here

07 August 2013 1,863 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View