21 Questions 40 Answers 0 Followers
Questions related from Debasish Paul
The protein purified by them is showing binding to the RNA. I have used exactly same lysis, washing and elution buffer. I have got purified very good purified protein.
04 April 2018 9,732 10 View
If one is interested to know whether a gene resides in loss of heterozygosity region of a cell line, what are the ways he/she can resolve it? Is there any database or online bioinformatic tool is...
11 November 2016 1,368 1 View
In research articles people often show meta analysis of a gene using NCBI GEO data sets or analysis, i want to know the procedure by which i can derive the information for a particular gene?
02 February 2015 2,523 3 View
I want to understand the importance of :Application note" section of a journal, Is it same as publishing a research article and have the same value (in terms of Impact factor of that journal) as...
01 January 2015 6,149 2 View
If a person x has done a research work that is presented (at past) somewhere (may be in form of poster or oral presentation) by some other person, will X be able to publish the similar research...
08 August 2014 7,200 1 View
If one is performing some experiments with cancerous cell lines, will it be a correct strategy to use HEK 293T as a normal cell line as a control?
03 March 2014 1,775 13 View
Can anybody inform me the concentration of L-Lysine and L-Arginine in normal RPMI and DMEM media?
02 February 2014 10,105 7 View
I am confused about how to calculate the fold change of transcript level by real time PCR, suppose in two conditions: control : GAPDH - 25 gene X- 18 Test : GAPDH - 23 gene X - 20 What will be...
01 January 2014 1,300 14 View
What are the experimental methods one can perform to find out whether a cell line is really resistance to a certain drug or not?
11 November 2013 1,245 3 View
Its a silly question.. one of my colleague asked me this question, but I couldn't answer so I am asking it here
08 August 2013 1,966 1 View
I am looking for a database or link that ll give information for different phosphatases..
07 July 2013 5,660 1 View
I am searching for a site or link where a student can see the upcoming internationals seminars and conferences that can help us to apply for our choice of conference..
07 July 2013 9,077 4 View
What is the status of p53 (gene as well as protein) in the lung cancer cell line H1299?
04 April 2013 3,522 9 View
I am observing cell death upon treatment of a a certain drug in A549 cells (lung cancer adherent). But I could not make out whether it is apoptosis, necrosis or autophagy. The cells are rounded...
03 March 2013 2,782 9 View
Generally I found that people use an ionic detergent for sample preparation of 2-DE , so what if i use ionic detergent like Tritonx 100 for 2-DE sample preparation?
03 March 2013 9,734 2 View
Can anyone help me with the amount of DNA, lipofectamine and proper vector control they used for a 96 well plate transfection assay.
02 February 2013 835 10 View
If a gene is GFP tagged, can I use it for an in vivo imaging experiment? If so, can anyone suggest some related protocols?
02 February 2013 3,224 12 View
Can anyone suggest the number of A549 cells and a procedure for doing in vivo (mice) tumor regression assays?
02 February 2013 4,887 5 View
I am working on the epigenetic silencing of a gene, upon 5-Aza treatment the transcript level of my gene of interest is going up four-fold compared to the untreated one. Unfortunately, the...
02 February 2013 8,788 14 View
I am measuring protein conc by Bradford assay, r2 value is >98, conc of protein prepared is 2ug/ul.
01 January 2013 3,540 7 View
I am transfecting these genes in MCF7 cells and try to evaluate the killing efficiency of the gene of interest?
01 January 2013 6,080 9 View
