Any online software or database available that provides information about loss of heterozygosity of a gene in a cell line?

11 November 2016 1 1K Report

If one is interested to know whether a gene resides in loss of heterozygosity region of a cell line, what are the ways he/she can resolve it? Is there any database or online bioinformatic tool is available for it??

Jose Castroesquivel

software & databases: DNACopy http://alternativeto.net/tag/dna-analysis/ http://alternativeto.net/browse/search?q=dna

http://nar.oxfordjournals.org/content/26/1/198.long

http://www.bio-rad.com/en-ca/applications-technologies/analysis-stem-cells

http://www1.biologie.uni-hamburg.de/b-online/library/genomeweb/GenomeWeb/human-gen-db-mutation.html

additional info: open and search "software" using Ctrl+F ("find")

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3737162/

http://digitalcommons.wustl.edu/cgi/viewcontent.cgi?article=5512&context=open_access_pubs

http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1000832

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725022/

Badges
Science topic

More Debasish Paul's questions See All

I have purified a protein using a protocol provided in a reasearch paper but this protein unable to bind to RNA (emsa) used as a positive control.Why?

The protein purified by them is showing binding to the RNA. I have used exactly same lysis, washing and elution buffer. I have got purified very good purified protein.

03 April 2018 9,776 10 View

How to use the NCBI GEO analysis/data-sets to find out expression profile of a particular gene?

In research articles people often show meta analysis of a gene using NCBI GEO data sets or analysis, i want to know the procedure by which i can derive the information for a particular gene?

01 February 2015 2,581 3 View

What is "Application Note" in a journal, does it have same value as research article publication?

I want to understand the importance of :Application note" section of a journal, Is it same as publishing a research article and have the same value (in terms of Impact factor of that journal) as...

31 December 2014 6,191 2 View

Ethical and technical issue

If a person x has done a research work that is presented (at past) somewhere (may be in form of poster or oral presentation) by some other person, will X be able to publish the similar research...

07 August 2014 7,248 1 View

Can we consider HEK 293T as a normal cell line?

If one is performing some experiments with cancerous cell lines, will it be a correct strategy to use HEK 293T as a normal cell line as a control?

02 March 2014 1,819 13 View

Concentration of L-Lysine and L-Arginine in normal RPMI and DMEM?

Can anybody inform me the concentration of L-Lysine and L-Arginine in normal RPMI and DMEM media?

01 February 2014 10,154 7 View

How to calculate real-time PCR data?

I am confused about how to calculate the fold change of transcript level by real time PCR, suppose in two conditions: control : GAPDH - 25 gene X- 18 Test : GAPDH - 23 gene X - 20 What will be...

31 December 2013 1,358 14 View

What are the ways a person can test the drug resistance for a particular cell line for a particular drug?

What are the experimental methods one can perform to find out whether a cell line is really resistance to a certain drug or not?

10 November 2013 1,299 3 View

How to remove the stains of developer from cloths?

Its a silly question.. one of my colleague asked me this question, but I couldn't answer so I am asking it here

07 August 2013 2,021 1 View

Is there any link or site where we can get the list of upcoming international seminars and conferences?

I am searching for a site or link where a student can see the upcoming internationals seminars and conferences that can help us to apply for our choice of conference..

06 July 2013 9,137 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View