08 August 2013 1 2K Report

Its a silly question.. one of my colleague asked me this question, but I couldn't answer so I am asking it here

More Debasish Paul's questions See All
I have purified a protein using a protocol provided in a reasearch paper but this protein unable to bind to RNA (emsa) used as a positive control.Why?

The protein purified by them is showing binding to the RNA. I have used exactly same lysis, washing and elution buffer. I have got purified very good purified protein.

03 April 2018 9,776 10 View

Any online software or database available that provides information about loss of heterozygosity of a gene in a cell line?

If one is interested to know whether a gene resides in loss of heterozygosity region of a cell line, what are the ways he/she can resolve it? Is there any database or online bioinformatic tool is...

10 November 2016 1,415 1 View

How to use the NCBI GEO analysis/data-sets to find out expression profile of a particular gene?

In research articles people often show meta analysis of a gene using NCBI GEO data sets or analysis, i want to know the procedure by which i can derive the information for a particular gene?

01 February 2015 2,581 3 View

What is "Application Note" in a journal, does it have same value as research article publication?

I want to understand the importance of :Application note" section of a journal, Is it same as publishing a research article and have the same value (in terms of Impact factor of that journal) as...

31 December 2014 6,191 2 View

Ethical and technical issue

If a person x has done a research work that is presented (at past) somewhere (may be in form of poster or oral presentation) by some other person, will X be able to publish the similar research...

07 August 2014 7,248 1 View

Can we consider HEK 293T as a normal cell line?

If one is performing some experiments with cancerous cell lines, will it be a correct strategy to use HEK 293T as a normal cell line as a control?

02 March 2014 1,819 13 View

Concentration of L-Lysine and L-Arginine in normal RPMI and DMEM?

Can anybody inform me the concentration of L-Lysine and L-Arginine in normal RPMI and DMEM media?

01 February 2014 10,154 7 View

How to calculate real-time PCR data?

I am confused about how to calculate the fold change of transcript level by real time PCR, suppose in two conditions: control : GAPDH - 25 gene X- 18 Test : GAPDH - 23 gene X - 20 What will be...

31 December 2013 1,358 14 View

What are the ways a person can test the drug resistance for a particular cell line for a particular drug?

What are the experimental methods one can perform to find out whether a cell line is really resistance to a certain drug or not?

10 November 2013 1,299 3 View

Is there any link or site where we can get the list of upcoming international seminars and conferences?

I am searching for a site or link where a student can see the upcoming internationals seminars and conferences that can help us to apply for our choice of conference..

06 July 2013 9,137 4 View

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Does anyone know where one can buy 8161 glass capillaries for making patch pipettes?

Warner used to sell 8161 glass, but Harvard Biosciences bought Warner and doesn’t appear to stock it anymore

04 June 2024 5,688 2 View

Problem: cell aggregation with Cytofix/Cytoperm BD Biosciences?

The challenge encountered lies in cellular aggregation and clumping subsequent to incubation with Cytofix/Cytoperm BD Biosciences. Despite attempts to mitigate the issue through pipetting, the...

12 March 2024 3,926 0 View

Isolation of rat mesenchymal stem cells (Fischer F455 rats) - protocol and surface markers?

Hi, We are trying to isolate rat MSCs from Fischer F455 rats. So far we tried a femur flush protocol to flush the bone marrow into a petri dish. However, we are wondering if we should do...

12 July 2023 4,684 0 View

Call of Editorial boards at Algerian Journal of Biosciences?

Call of Edotrial boards in Algerian Journal of Biosciences About more informations please visit the journal website https://www.journal.acse.science/index.php/ajb/index If you are interested...

17 April 2023 3,999 0 View

Philosophically, are stars alive?

In the field of biosciences we often get in contact with the questions: Are viruses alive, a form of life? Is fire a form of life? Stars also burn fuel and stay stable until their fuel is burned...

16 April 2023 7,233 1 View

Where to purchase viruses other than ATCC?

I have been looking to expand our virology department here for a year since joining my current institution. I have only ever purchased viruses from Carolina Biosciences (bacteriophage) and ATCC...

12 January 2023 4,248 2 View

Is it true that the molecular weight of asyn protein is 25kd in westen-blot ?

The protein was extracted from mouse tissue(brain)，marker is Thermo 26619. The ladder was about in 25kd.And the antibody is BD Bioscience#asyn antibody. I'm not sure if it was α-synuclein.

08 December 2022 9,772 4 View

How do I sequence my isolated virus?

I've grown my influenza straind in MDCK cells and have isolated them. Now I have to sequence my virus, my lab uses Eton Biosciences. Do I send in primers for each gene? Do I extract RNA from the...

18 May 2022 2,592 0 View

My TCF/LEF Reporter - HEK293 Cell Line (Wnt Signaling Pathway) is struggling to grow upon thawing. Has anyone had similar issue with this cell line?

The reporter cell line has been cultured following the suppliers recommendation (see below): Thaw Medium 1 (BPS Bioscience #60187): MEM medium (Thermo Fisher, #11095098) supplemented with 10% FBS...

09 January 2022 3,186 0 View

Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences?

Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences and if so could you please tell me if there was any problems regarding communications with the...

05 October 2020 1,928 2 View

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