I have purified a protein using a protocol provided in a reasearch paper but this protein unable to bind to RNA (emsa) used as a positive control.Why?

The protein purified by them is showing binding to the RNA. I have used exactly same lysis, washing and elution buffer. I have got purified very good purified protein.

03 April 2018 9,634 10 View

Any online software or database available that provides information about loss of heterozygosity of a gene in a cell line?

If one is interested to know whether a gene resides in loss of heterozygosity region of a cell line, what are the ways he/she can resolve it? Is there any database or online bioinformatic tool is...

10 November 2016 1,271 1 View

How to use the NCBI GEO analysis/data-sets to find out expression profile of a particular gene?

In research articles people often show meta analysis of a gene using NCBI GEO data sets or analysis, i want to know the procedure by which i can derive the information for a particular gene?

01 February 2015 2,395 3 View

What is "Application Note" in a journal, does it have same value as research article publication?

I want to understand the importance of :Application note" section of a journal, Is it same as publishing a research article and have the same value (in terms of Impact factor of that journal) as...

31 December 2014 6,059 2 View

Can we consider HEK 293T as a normal cell line?

If one is performing some experiments with cancerous cell lines, will it be a correct strategy to use HEK 293T as a normal cell line as a control?

02 March 2014 1,677 13 View

Concentration of L-Lysine and L-Arginine in normal RPMI and DMEM?

Can anybody inform me the concentration of L-Lysine and L-Arginine in normal RPMI and DMEM media?

01 February 2014 10,015 7 View

How to calculate real-time PCR data?

I am confused about how to calculate the fold change of transcript level by real time PCR, suppose in two conditions: control : GAPDH - 25 gene X- 18 Test : GAPDH - 23 gene X - 20 What will be...

31 December 2013 1,167 14 View

What are the ways a person can test the drug resistance for a particular cell line for a particular drug?

What are the experimental methods one can perform to find out whether a cell line is really resistance to a certain drug or not?

10 November 2013 1,163 3 View

How to remove the stains of developer from cloths?

Its a silly question.. one of my colleague asked me this question, but I couldn't answer so I am asking it here

07 August 2013 1,863 1 View

Is there any link or site where we can get the list of upcoming international seminars and conferences?

I am searching for a site or link where a student can see the upcoming internationals seminars and conferences that can help us to apply for our choice of conference..

06 July 2013 8,967 4 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

Why is ethics significant in business and how should one control unethical behavior ?

1. The significance of ethics to business and how to control bad behavior.

21 February 2021 882 14 View

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem?

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem.

21 February 2021 9,133 2 View

Discuss the extent to which morality in African traditional societies can be classified under the Natural Law theory of morality?

How can one classified African traditional societies under natural law

18 February 2021 7,334 8 View

Is it appropriate to use AIDA model (Assessment of Identity Development in Adolescence) on a non-clinical sample?

I am researching emotion regulation and identity development in adolescence. I've come across several inventories for assessing identity development and it seems like the AIDA scale is the most...

16 February 2021 1,261 1 View

How to derive equations for a resonant buck converter with ZVS?

I am not understanding how each author is deriving the equations and the working principle. Individual authors are giving their own working principle explanation for different circuit topology....

12 February 2021 496 1 View

Which ethical metrics is expected from an academic who is the owner or the creator of the measuring instrument?

Which ethical metrics is expected from an academic who is the owner or the creator of the instrument? The measurement tool will be applied to adults as an online form and a snowball sample will...

01 February 2021 9,188 3 View

Help me conduct a neuroscience experiment for my master's thesis?

22 January 2021 9,994 5 View

Genus name priority from the same publication (Zoology)?

I came across a recent taxonomic work, where two genera were synonymized. Both genera were published in a paper in 1922 by the original author who revised the family at that time. The original...

19 January 2021 1,920 4 View