I am currently looking for a near diploid human cancer cell line, such as HCT116 and U87-MG. Preferably XY with modal chromosome # between 40-50, "normal" DNA repair pathways, and able to easily be transfected and cloned. Does anyone have any suggestions?
Dear Ian, You can please follow the links below to obtain certain interesting informations:-
1. http://www.nature.com/jid/journal/v114/n3/abs/5603293a.html
2. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2818350/
3. http://www.pnas.org/content/95/23/13692.long
Hi Ian,
It actually depends on what are you trying to study with these cell lines.
Although HCT116 cells are mismatch repair deficient, if you are trying to study something like Double-strand breaks, they should be a good choice. They are p53 proficient and show a normal ATM-mediated G1/S block. Although if you are trying to study SSBs, they might not be that helpful. So as I said it all depends on what you are trying to study.
Good luck with your research!
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