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Questions related from Ian Cartwright
I am currently looking for a near diploid human cancer cell line, such as HCT116 and U87-MG. Preferably XY with modal chromosome # between 40-50, "normal" DNA repair pathways, and able to easily...
07 July 2015 4,665 2 View
Hello All, I am trying to do a simple fluorescence plus Giemsa stain/acridine orange differential stain for chromosomes. I have had great success in the past using MEF/CHO/and various cancer cell...
12 December 2014 868 2 View
Hello All, I am currently struggling with getting a Gibson Assembly to work. I am trying to insert a 2.5 Kb insert into a roughly 12 Kb plasmid (modified pLEX-MCS). I have had the G.A work in the...
12 December 2014 4,248 7 View
Afternoon everyone, I am currently using Q5 polymerase from NEB to add restriction sites to an insert in a pBabepuro plasmid (~5400 Kb vector and 2.2 Kb insert). I have designed the primers to...
10 October 2014 4,519 4 View
Hello, Addgene does not have the complete sequence published. Also, does anyone know if myc is truncated on both the N and C terminus, a primer designed to target the start of the myc CDS,...
09 September 2014 1,447 1 View
I have a plasmid with an I-SceI restriction site which I want to only cut once, which I do not believe can happen with an I-SceI expression vector.
11 November 2013 6,479 0 View
I have been running several western blots over the past few days and have had the same issue where large proteins seem to stain nicely where as the smaller (~50 Kda) stain very poorly. I am using...
06 June 2013 9,747 4 View
