07 July 2017 1 6K Report

Hello, does anybody has any electroporation protocol for transfecting the tumoral cell lines MDA-MB 231 and SK-MEL 28 or others?

Many thanks! 

More Martin Williams's questions See All
Single E coli colony transformation?

Is there any protocol to make chemically competent E coli from a single colony and transform it? Any buffer recipe? Many thanks

02 March 2018 9,760 0 View

Electrocompetent bacteria storage at -20?

Hello, I'm trying to make electrocompetent bacteria and store them at -20 degrees as I don't have -80 freezers. Is there any protocol? Should I use 50% glycerol? Thanks

06 July 2017 3,155 1 View

Eukaryote Promoter consensus sequences analysis ?

Is there any easy-to-use software to analyze core promoter regions, (TATA, INR, DPE) and other distal promoter regions or enhancers ? Any paper or review of eukaryotes promoters? Many thanks! Martin 

10 November 2016 1,077 4 View

DNA "Pellet-Paint" coprecipitant or similar composition?

Hello!, I want to see the DNA pellet, is there any dye or something to paint the pellet and see it ?  Thanks! Martin. 

09 October 2016 8,303 9 View

Tumoral cell line intradermical nude mice inoculation?

Hello, I need to generate tumors in nude mice with the cell lines SK MEL 28 (melanoma) and MDA MB 231 (breast cancer), both human tumoral cell lines. How many cells do i have to inject...

05 June 2016 3,406 4 View

PEI transfection of SK-MEL 28?

Im trying to transfect the melanoma cell line SK-MEL 28 with PEI, has anyone transfected this line before with PEI? Any PEI transfection tips?  Many thanks!

02 March 2016 5,312 4 View

Has anybody got any protocol to make competent lyophilized E coli?

Hello, Has anybody got any protocol to make competent lyophilized E coli?

10 November 2015 2,062 0 View

Add ssDNA to a Gold Nanoparticle?

Im trying to make a Gold nanoparticle with ssDNA to use as a probe. Is there any way to make this "coating" in a relative simple way?  Many thanks !

09 October 2015 3,298 1 View

Protocols to liofilize DNA?

Im trying to liofilize DNA, is there any protocol i should use ?  Thanks. Martin

05 June 2015 8,661 3 View

What is an equivalent alternative reagents composition like the Zymo Mix&Go?

Searching the web i found that Zymo Research makes a kit for making competent cells and transforming them in aprox 20 seconds without incubating the mix on ice for 30 minutes, without heat-shock...

04 May 2015 3,621 4 View

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