Add ssDNA to a Gold Nanoparticle?

10 October 2015 1 3K Report

Im trying to make a Gold nanoparticle with ssDNA to use as a probe. Is there any way to make this "coating" in a relative simple way?

Many thanks !

Rostislav Váňa

We produced such a protocol in here http://pubs.acs.org/doi/abs/10.1021/acsami.5b01191

Badges
Science topic

More Martin Williams's questions See All

Single E coli colony transformation?

Is there any protocol to make chemically competent E coli from a single colony and transform it? Any buffer recipe? Many thanks

02 March 2018 9,760 0 View

Mammalian cell culture electroporation?

Hello, does anybody has any electroporation protocol for transfecting the tumoral cell lines MDA-MB 231 and SK-MEL 28 or others? Many thanks!

06 July 2017 6,457 1 View

Electrocompetent bacteria storage at -20?

Hello, I'm trying to make electrocompetent bacteria and store them at -20 degrees as I don't have -80 freezers. Is there any protocol? Should I use 50% glycerol? Thanks

06 July 2017 3,155 1 View

Eukaryote Promoter consensus sequences analysis ?

Is there any easy-to-use software to analyze core promoter regions, (TATA, INR, DPE) and other distal promoter regions or enhancers ? Any paper or review of eukaryotes promoters? Many thanks! Martin

10 November 2016 1,077 4 View

DNA "Pellet-Paint" coprecipitant or similar composition?

Hello!, I want to see the DNA pellet, is there any dye or something to paint the pellet and see it ? Thanks! Martin.

09 October 2016 8,303 9 View

Tumoral cell line intradermical nude mice inoculation?

Hello, I need to generate tumors in nude mice with the cell lines SK MEL 28 (melanoma) and MDA MB 231 (breast cancer), both human tumoral cell lines. How many cells do i have to inject...

05 June 2016 3,406 4 View

PEI transfection of SK-MEL 28?

Im trying to transfect the melanoma cell line SK-MEL 28 with PEI, has anyone transfected this line before with PEI? Any PEI transfection tips? Many thanks!

02 March 2016 5,312 4 View

Has anybody got any protocol to make competent lyophilized E coli?

Hello, Has anybody got any protocol to make competent lyophilized E coli?

10 November 2015 2,062 0 View

Protocols to liofilize DNA?

Im trying to liofilize DNA, is there any protocol i should use ? Thanks. Martin

05 June 2015 8,661 3 View

What is an equivalent alternative reagents composition like the Zymo Mix&Go?

Searching the web i found that Zymo Research makes a kit for making competent cells and transforming them in aprox 20 seconds without incubating the mix on ice for 30 minutes, without heat-shock...

04 May 2015 3,621 4 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

How to build my own lab made four point probe set up?

Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...

30 July 2024 906 2 View