12 Questions 16 Answers 0 Followers
Questions related from Martin Williams
Is there any protocol to make chemically competent E coli from a single colony and transform it? Any buffer recipe? Many thanks
03 March 2018 9,712 0 View
Hello, does anybody has any electroporation protocol for transfecting the tumoral cell lines MDA-MB 231 and SK-MEL 28 or others? Many thanks!
07 July 2017 6,396 1 View
Hello, I'm trying to make electrocompetent bacteria and store them at -20 degrees as I don't have -80 freezers. Is there any protocol? Should I use 50% glycerol? Thanks
07 July 2017 3,100 1 View
Is there any easy-to-use software to analyze core promoter regions, (TATA, INR, DPE) and other distal promoter regions or enhancers ? Any paper or review of eukaryotes promoters? Many thanks! Martin
11 November 2016 999 4 View
Hello!, I want to see the DNA pellet, is there any dye or something to paint the pellet and see it ? Thanks! Martin.
10 October 2016 8,252 9 View
Hello, I need to generate tumors in nude mice with the cell lines SK MEL 28 (melanoma) and MDA MB 231 (breast cancer), both human tumoral cell lines. How many cells do i have to inject...
06 June 2016 3,338 4 View
Im trying to transfect the melanoma cell line SK-MEL 28 with PEI, has anyone transfected this line before with PEI? Any PEI transfection tips? Many thanks!
03 March 2016 5,261 4 View
Hello, Has anybody got any protocol to make competent lyophilized E coli?
11 November 2015 2,007 0 View
Im trying to make a Gold nanoparticle with ssDNA to use as a probe. Is there any way to make this "coating" in a relative simple way? Many thanks !
10 October 2015 3,234 1 View
Im trying to liofilize DNA, is there any protocol i should use ? Thanks. Martin
06 June 2015 8,604 3 View
Searching the web i found that Zymo Research makes a kit for making competent cells and transforming them in aprox 20 seconds without incubating the mix on ice for 30 minutes, without heat-shock...
05 May 2015 3,543 4 View
I have a plasmid resuspended in TE and i have to digest it, do i have to do an ethanol precipitation first? Besides that, should i resuspend my DNA pellets in TE, Tris 10 mM or Roux if i need to...
04 April 2015 7,119 8 View
